NDLI: Rapid De Novo Centromere Formation Occurs Independently of Heterochromatin Protein 1 in C. elegans Embryos

Content Provider	PubMed Central
Author	Yuen, Karen W. Y. Nabeshima, Kentaro Karen, Oegema Desai, Arshad
Copyright Year	2011
Abstract	DNA injected into the C. elegans germline forms extrachromosomal arrays that segregate during cell division [1, 2]. The mechanisms underlying array formation and segregation are not known. Here, we show that extrachromosomal arrays form de novo centromeres at high frequency, providing unique access to a process that occurs with extremely low frequency in other systems [3–8]. De novo centromerized arrays recruit centromeric chromatin and kinetochore proteins and autonomously segregate on the spindle. Live imaging following DNA injection revealed that arrays form after oocyte fertilization via homologous recombination and non-homologous end joining. Individual arrays gradually transition from passive inheritance to active segregation during the early embryonic divisions. The Heterochromatin Protein 1 (HP1) family proteins HPL-1 and HPL-2 are dispensable for de novo centromerization even though arrays become strongly enriched for the heterochromatin-associated H3K9me3 modification over time. Partial inhibition of HP1 family proteins accelerates the acquisition of segregation competence. In addition to reporting the first direct visualization of new centromere formation in living cells, these findings reveal that naked DNA rapidly builds de novo centromeres in C. elegans embryos in an HP1-independent manner, and suggest that, rather than being a prerequisite, HP1-dependent heterochromatin antagonizes de novo centromerization.
Related Links	http://dx.doi.org/10.1016/j.cub.2011.09.016
Ending Page	1807
Page Count	8
Starting Page	1800
File Format	PDF
ISSN	09609822
e-ISSN	18790445
Journal	Current biology : CB
Issue Number	21
Volume Number	21
Language	English
Publisher Date	2011-11-01
Access Restriction	Open
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Biochemistry, Genetics and Molecular Biology Agricultural and Biological Sciences

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

EGO-1, a Putative RNA-Dependent RNA Polymerase, Is Required for Heterochromatin Assembly on Unpaired DNA during C. elegans Meiosis

The C. elegans dosage compensation complex propagates along chromatin dynamically and independently of X-chromosome sequence

Condensin and the spindle midzone prevent cytokinesis failure induced by chromatin bridges in C. elegans embryos

The P4-ATPase TAT-5 inhibits the budding of extracellular vesicles in C. elegans embryos

The role of heterochromatin in centromere function

Overexpressing Centriole-Replication Proteins In Vivo Induces Centriole Overduplication and De Novo Formation

piRNA production requires heterochromatin formation in Drosophila

Identification of a Polo-like Kinase 4-Dependent Pathway for De Novo Centriole Formation

ENDOCYTOSIS GENES FACILITATE PROTEIN AND MEMBRANE TRANSPORT IN C. ELEGANS SENSORY CILIA

Rapid De Novo Centromere Formation Occurs Independently of Heterochromatin Protein 1 in C. elegans Embryos

Similar Documents

EGO-1, a Putative RNA-Dependent RNA Polymerase, Is Required for Heterochromatin Assembly on Unpaired DNA during C. elegans Meiosis

The C. elegans dosage compensation complex propagates along chromatin dynamically and independently of X-chromosome sequence

Condensin and the spindle midzone prevent cytokinesis failure induced by chromatin bridges in C. elegans embryos

The P4-ATPase TAT-5 inhibits the budding of extracellular vesicles in C. elegans embryos

The role of heterochromatin in centromere function

Overexpressing Centriole-Replication Proteins In Vivo Induces Centriole Overduplication and De Novo Formation

piRNA production requires heterochromatin formation in Drosophila

Identification of a Polo-like Kinase 4-Dependent Pathway for De Novo Centriole Formation

ENDOCYTOSIS GENES FACILITATE PROTEIN AND MEMBRANE TRANSPORT IN C. ELEGANS SENSORY CILIA

Rapid De Novo Centromere Formation Occurs Independently of Heterochromatin Protein 1 in C. elegans Embryos