NDLI: Fine-Tuning Control of phoBR Expression in Vibrio cholerae by Binding of PhoB to Multiple Pho Boxes ▿

Content Provider	PubMed Central
Author	Diniz, Michelle Menezes Passos Goulart, Carolina Lage Barbosa, Livia Carvalho Farache, Júlia Lery, Letícia Miranda Santos Pacheco, Ana Beatriz Furlanetto Bisch, Paulo Mascarello Krüger, Wanda Maria De Almeida Von
Copyright Year	2011
Abstract	The control of Vibrio cholerae phoBR expression by PhoB involves its binding to Pho boxes at −35 (box 1), −60 (box 2), and −80 (box 3) from the putative phoB translation start site. These loci were located in the sense (box 1) and antisense (boxes 2 and 3) strands of the phoBR regulatory region, and PhoB binds to these individual boxes with distinct affinities. Fusions of sequences containing different combinations of these boxes upstream of the lacZ reporter in a plasmid demonstrated that only those carrying boxes 1, 2, and 3, or 1 alone, activated transcription under inorganic phosphate (Pi) limitation. When a fragment, including only boxes 1 and 2, was fused to lacZ, expression was no longer induced by low Pi, suggesting a repressive role for PhoB∼box2 (PhoB bound to box 2) over the transcriptional activity induced by PhoB∼box1. The similarity between lacZ expression levels from promoter fragments containing the three boxes or box 1 alone showed that PhoB∼box3 eliminated the repressive effect imposed by PhoB∼box2 on phoBR transcription. Complementation assays with a phoBR-containing plasmid demonstrated that the 234-bp promoter fragment carrying the three boxes is absolutely required for operon expression in Vibrio cholerae ΔphoBR cells. This was observed under Pi abundance, when phoBR was expressed at a basal level and, also in low Pi conditions, when Pho regulon genes were fully expressed. Thus, under Pi limitation, PhoB exerts dual regulatory functions by binding sequentially distinct Pho boxes to enable the fine-tuning and precise control of phoBR expression in V. cholerae cells.
Related Links	http://dx.doi.org/10.1128/JB.06015-11
Starting Page	6929
File Format	PDF
ISSN	10985530
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	24
Volume Number	193
Language	English
Publisher	American Society for Microbiology
Publisher Date	2011-12-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

PhoB regulates both environmental and virulence gene expression in Vibrio cholerae

PhoB Regulates Motility, Biofilms, and Cyclic di-GMP in Vibrio cholerae ▿

The PhoB regulatory system modulates biofilm formation and stress response in El Tor biotype Vibrio cholerae

Fine-Tuning of Galactoglucan Biosynthesis in Sinorhizobium meliloti by Differential WggR (ExpG)-, PhoB-, and MucR-Dependent Regulation of Two Promoters ▿

Fine Structure of Vibrio cholerae During Toxin Production

Characterization of Enhancer Binding by the Vibrio cholerae Flagellar Regulatory Protein FlrC

CONTRIBUTIONS TO THE KNOWLEDGE OF VIBRIO CHOLERAE I. FERMENTATION OF CARBOHYDRATES AND POLYATOMIC ALCOHOLS BY VIBRIO CHOLERAE

Bicarbonate Increases Binding Affinity of Vibrio cholerae ToxT to Virulence Gene Promoters

Differential Regulation of Multiple Flagellins in Vibrio cholerae

Fine-Tuning Control of phoBR Expression in Vibrio cholerae by Binding of PhoB to Multiple Pho Boxes ▿

Similar Documents

PhoB regulates both environmental and virulence gene expression in Vibrio cholerae

PhoB Regulates Motility, Biofilms, and Cyclic di-GMP in Vibrio cholerae ▿

The PhoB regulatory system modulates biofilm formation and stress response in El Tor biotype Vibrio cholerae

Fine-Tuning of Galactoglucan Biosynthesis in Sinorhizobium meliloti by Differential WggR (ExpG)-, PhoB-, and MucR-Dependent Regulation of Two Promoters ▿

Fine Structure of Vibrio cholerae During Toxin Production

Characterization of Enhancer Binding by the Vibrio cholerae Flagellar Regulatory Protein FlrC

CONTRIBUTIONS TO THE KNOWLEDGE OF VIBRIO CHOLERAE I. FERMENTATION OF CARBOHYDRATES AND POLYATOMIC ALCOHOLS BY VIBRIO CHOLERAE

Bicarbonate Increases Binding Affinity of Vibrio cholerae ToxT to Virulence Gene Promoters

Differential Regulation of Multiple Flagellins in Vibrio cholerae

Fine-Tuning Control of phoBR Expression in Vibrio cholerae by Binding of PhoB to Multiple Pho Boxes ▿