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| Content Provider | PubMed Central |
|---|---|
| Author | Reha-krantz, Linda J. Hariharan, Chithra Subuddhi, Usharani Xia, Shuangluo Zhao, Chao Beckman, Jeff Christian, Thomas Konigsberg, William |
| Abstract | The adenine base analog 2-aminopurine (2AP) is a potent base substitution mutagen in prokaryotes because of increased ability to form a mutagenic base pair with an incoming dCTP. Despite more than 50 years of research, the structure of the 2AP-C base pair remains unclear. We report the structure of the 2AP-dCTP base pair formed within the polymerase active site of the RB69 Y567A-DNA polymerase. A modified wobble 2AP-C base pair was detected with one H-bond between the N1 of 2AP and a proton from the C4 amino group of cytosine and an apparent bifurcated H-bond between a proton on the 2-amino group of 2-aminopurine and the ring N3 and O2 of cytosine. Interestingly, a primer-terminal region rich in AT base pairs, compared to GC base pairs, facilitated dCTP binding opposite template 2AP. We propose that increased flexibility of the nucleotide binding pocket formed in the Y567A-DNA polymerase and increased ‘breathing’ at the primer-terminal junction of A+T-rich DNA facilitates dCTP binding opposite template 2AP. Thus, interactions between DNA polymerase residues with a dynamic primer-terminal junction play a role in determining base selectivity within the polymerase active site of RB69 DNA polymerase. |
| Related Links | http://dx.doi.org/10.1021/bi2014618 |
| Starting Page | 10136 |
| File Format | |
| ISSN | 15204995 |
| e-ISSN | 15204995 |
| Journal | Biochemistry |
| Issue Number | 46 |
| Volume Number | 50 |
| Language | English |
| Publisher Date | 2011-11-22 |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry |
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