NDLI: An unusually long poly(purine)-poly(pyrimidine) sequence is located upstream from the human thyroglobulin gene.

Content Provider	PubMed Central
Author	Christophe, D. Cabrer, B. Bacolla, A. Targovnik, H. Vassart, G. Pohl, V.
Abstract	A region of human genomic DNA encompassing the 5' end of the thyroglobulin gene has been sequenced and the position of the transcriptional start site has been determined. The 5' non-translated portion of the mRNA displays a quasi-palindromic sequence which could allow this region to adopt a hairpin structure. The first exon of the gene encodes a 19 amino-acids signal peptide and the 3 first amino acids of the mature protein. Apart from the canonical TATA-Box and from a CAAT-Box homology, the promoter region contains a 209 bp-long poly(purine)-poly (pyrimidine) sequence located between positions-512 and -304 relative to the transcription start. When contained in a supercoiled plasmid, this sequence exhibits sensitivity to S1 nuclease at two distinct positions. A precise mapping of the borders of the sensitive regions was achieved by extending primers from both ends of the sequence after digestion by the enzyme. The resulting data can be explained by a model involving the formation of a triple helix structure.
Starting Page	5127
File Format	PDF
ISSN	13624962
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	14
Volume Number	13
Language	English
Publisher Date	1985-07-25
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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An unusually long poly(purine)-poly(pyrimidine) sequence is located upstream from the human thyroglobulin gene.