NDLI: Induction of erythropoiesis using human vascular networks genetically engineered for controlled erythropoietin release

Content Provider	PubMed Central
Author	Lin, Ruei-zeng Alexandra, Dreyzin Aamodt, Kristie Li, Dan Jaminet, Shou-ching S. Dudley, Andrew C. Melero-martin, Juan M.
Copyright Year	2011
Abstract	For decades, autologous ex vivo gene therapy has been postulated as a potential alternative to parenteral administration of recombinant proteins. However, achieving effective cellular engraftment of previously retrieved patient cells is challenging. Recently, our ability to engineer vasculature in vivo has allowed for the introduction of instructions into tissues by genetically modifying the vascular cells that build these blood vessels. In the present study, we genetically engineered human blood–derived endothelial colony-forming cells (ECFCs) to express erythropoietin (EPO) under the control of a tetracycline-regulated system, and generated subcutaneous vascular networks capable of systemic EPO release in immunodeficient mice. These ECFC-lined vascular networks formed functional anastomoses with the mouse vasculature, allowing direct delivery of recombinant human EPO into the bloodstream. After activation of EPO expression, erythropoiesis was induced in both normal and anemic mice, a process that was completely reversible. This approach could relieve patients from frequent EPO injections, reducing the medical costs associated with the management of anemia. We propose this ECFC-based gene-delivery strategy as a viable alternative technology when routine administration of recombinant proteins is needed.
Related Links	http://dx.doi.org/10.1182/blood-2011-08-372946
Ending Page	5428
Page Count	9
Starting Page	5420
File Format	PDF
ISSN	00064971
e-ISSN	15280020
Journal	Blood
Issue Number	20
Volume Number	118
Language	English
Publisher	American Society of Hematology
Publisher Date	2011-11-17
Access Restriction	Open
Rights Holder	American Society of Hematology
Subject Keyword	Immunology Cell Biology Biochemistry Hematology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Hematology Biochemistry Immunology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Protein kinase D-HDAC5 signaling regulates erythropoiesis and contributes to erythropoietin cross-talk with GATA1

Gene induction and repression during terminal erythropoiesis are mediated by distinct epigenetic changes

Human extramedullary bone marrow in mice: a novel in vivo model of genetically controlled hematopoietic microenvironment

Erythroblastic islands: niches for erythropoiesis

Cocaine-mediated induction of platelet-derived growth factor: implication for increased vascular permeability

Critical band 3 multiprotein complex interactions establish early during human erythropoiesis

Evidence for ineffective erythropoiesis in severe sickle cell disease

Regulated expression of microRNAs-126/126* inhibits erythropoiesis from human embryonic stem cells

Selective induction of endothelial P2Y6 nucleotide receptor promotes vascular inflammation

Induction of erythropoiesis using human vascular networks genetically engineered for controlled erythropoietin release

Similar Documents

Protein kinase D-HDAC5 signaling regulates erythropoiesis and contributes to erythropoietin cross-talk with GATA1

Gene induction and repression during terminal erythropoiesis are mediated by distinct epigenetic changes

Human extramedullary bone marrow in mice: a novel in vivo model of genetically controlled hematopoietic microenvironment

Erythroblastic islands: niches for erythropoiesis

Cocaine-mediated induction of platelet-derived growth factor: implication for increased vascular permeability

Critical band 3 multiprotein complex interactions establish early during human erythropoiesis

Evidence for ineffective erythropoiesis in severe sickle cell disease

Regulated expression of microRNAs-126/126* inhibits erythropoiesis from human embryonic stem cells

Selective induction of endothelial P2Y6 nucleotide receptor promotes vascular inflammation

Induction of erythropoiesis using human vascular networks genetically engineered for controlled erythropoietin release