NDLI: Two-Photon Fluorescence Vascular Bioimaging with New Bioconjugate Probes Selective Towards the Vascular Endothelial Growth Factor Receptor 2

Content Provider	PubMed Central
Author	Andrade, Carolina D. Yanez, Ciceron O. Ahn, Hyo-yang Urakami, Takeo Bondar, Mykhailo V. Komatsu, Masanobu Belfield, Kevin D.
Abstract	We report the synthesis and characterization of two amine reactive fluorescent dyes with efficient two-photon absorption (2PA) properties and high fluorescence quantum yields. Bioconjugation of these dyes with the DC-101 antibody proved to be useful for selectively imaging the vascular endothelial growth factor receptor 2 (VEGFR-2) in cells expressing this receptor in vitro and in “whole” mounted excised tumors (ex vivo) by two-photon fluorescence microscopy (2PFM). The penetration depths reached within the tumors by 2PFM was over 800 μm. In addition, the concentration of dye required for incubation of these bioconjugates was in the picomolar domain, the probes possessed very good photostability, and the 2PFM setup did not require any additional means of increasing the collection efficiencies of fluorescent photons to achieve the relatively deep tissue imaging that was realized, due, in large part, to the favorable photophysical properties of the new probes.
Related Links	http://dx.doi.org/10.1021/bc200299z
Ending Page	2071
Page Count	12
Starting Page	2060
File Format	PDF
ISSN	10431802
e-ISSN	15204812
Journal	Bioconjugate chemistry
Issue Number	10
Volume Number	22
Language	English
Publisher Date	2011-10-19
Access Restriction	Open
Subject Keyword	Biotechnology Organic Chemistry Bioengineering Pharmacology Pharmaceutical Science Biomedical Engineering Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Pharmacology Bioengineering Biomedical Engineering Biotechnology Pharmaceutical Science

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