NDLI: Anthocyanin production as a potential visual selection marker during plant transformation

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Anthocyanin production as a potential visual selection marker during plant transformation

Content Provider	PubMed Central
Author	Kortstee, A. J. Khan, S. A. Helderman, C. Trindade, L. M. Wu, Y. Visser, R. G. F. Brendolise, C. Allan, A. Schouten, H. J. Jacobsen, E.
Abstract	A mutant allele of the transcription factor gene MYB10 from apple induces anthocyanin production throughout the plant. This gene, including its upstream promoter, gene coding region and terminator sequence, was introduced into apple, strawberry and potato plants to determine whether it could be used as a visible selectable marker for plant transformation as an alternative to chemically selectable markers, such as kanamycin resistance. After transformation, red coloured calli, red shoots and red well-growing plants were scored. Red and green shoots were harvested from apple explants and examined for the presence of the MYB10 gene by PCR analysis. Red shoots of apple explants always contained the MYB10 gene but not all MYB10 containing shoots were red. Strawberry plants transformed with the MYB10 gene showed anthocyanin accumulation in leaves and roots. No visible accumulation of anthocyanin could be observed in potato plants grown in vitro, even the ones carrying the MYB10 gene. However, acid methanol extracts of potato shoots or roots carrying the MYB10 gene contained up to four times higher anthocyanin content than control plants. Therefore anthocyanin production as result of the apple MYB10 gene can be used as a selectable marker for apple, strawberry and potato transformation, replacing kanamycin resistance.
Related Links	http://dx.doi.org/10.1007/s11248-011-9490-1
Ending Page	1264
Page Count	12
Starting Page	1253
File Format	PDF
ISSN	09628819
e-ISSN	15739368
Journal	Transgenic Research
Issue Number	6
Volume Number	20
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-12-01
Access Restriction	Open
Rights Holder	Springer Netherlands
Subject Keyword	Biotechnology Agronomy and Crop Science Animal Science and Zoology Genetics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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