Loading...
Please wait, while we are loading the content...
Similar Documents
Laser crosslinking of E. coli RNA polymerase and T7 DNA.
| Content Provider | PubMed Central |
|---|---|
| Author | Harrison, C. A. Turner, D. H. Hinkle, D. C. |
| Abstract | The first photochemical crosslinking of a protein to a nucleic acid using laser excitation is reported. A single, 120 mJ, 20 ns pulse at 248 nm crosslinks about 10% of bound E. coli RNA polymerase to T7 DNA under the conditions studied. The crosslinking yield depends on mercaptoethanol concentration, and is a linear function of laser intensity. The protein subunits crosslinked to DNA are beta, beta' and sigma. |
| Ending Page | 2414 |
| Starting Page | 2399 |
| File Format | |
| ISSN | 13624962 |
| e-ISSN | 13624962 |
| Journal | Nucleic Acids Research |
| Issue Number | 7 |
| Volume Number | 10 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics |
