NDLI: A high-throughput microfluidic real-time gene expression living cell array †

Please wait, while we are loading the content...

A high-throughput microfluidic real-time gene expression living cell array †

Content Provider	PubMed Central
Author	King, Kevin R. Wang, Sihong Irimia, Daniel Jayaraman, Arul Toner, Mehmet Yarmush, Martin L.
Copyright Year	2007
Abstract	The dynamics of gene expression are fundamental to the coordination of cellular responses. Measurement of temporal gene expression patterns is currently limited to destructive low-throughput techniques such as northern blotting, reverse transcription polymerase chain reaction (RT-PCR), and DNA microarrays. We report a scalable experimental platform that combines microfluidic addressability with quantitative live cell imaging of fluorescent protein transcriptional reporters to achieve real-time characterization of gene expression programs in living cells. Integrated microvalve arrays control row-seeding and column-stimulation of 256 nanoliter-scale bioreactors to create a high density matrix of stimulus–response experiments. We demonstrate the approach in the context of hepatic inflammation by acquiring ~5000 single-time-point measurements in each automated and unattended experiment. Experiments can be assembled in hours and perform the equivalent of months of conventional experiments. By enabling efficient investigation of dynamic gene expression programs, this technology has the potential to make significant impacts in basic science, drug development, and clinical medicine.
Related Links	http://dx.doi.org/10.1039/b612516f
Ending Page	85
Page Count	9
Starting Page	77
File Format	PDF
ISSN	14730197
e-ISSN	14730189
Journal	Lab on a chip
Issue Number	1
Volume Number	7
Language	English
Publisher Date	2007-01-01
Access Restriction	Open
Subject Keyword	Biochemistry Bioengineering Chemistry(all) Biomedical Engineering Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

High-throughput tracking of single yeast cells in a microfluidic imaging matrix

A microfluidic chip for real-time studies of the volume of single cells†

Microfluidic heart on a chip for higher throughput pharmacological studies

Engineering anastomosis between living capillary networks and endothelial cell-lined microfluidic channels

A microfluidic processor for gene expression profiling of single human embryonic stem cells

The single-cell chemostat: an agarose-based, microfluidic device for high-throughput, single-cell studies of bacteria and bacterial communities

Design, Fabrication and Implementation of a Novel Multi Parameter Control Microfluidic Platform for Three-Dimensional Cell Culture and Real-Time Imaging

A microfluidic platform for chemical stimulation and real time analysis of catecholamine secretion from neuroendocrine cells

Methods to array photonic crystal microcavities for high throughput high sensitivity biosensing on a silicon-chip based platform

A high-throughput microfluidic real-time gene expression living cell array †

Similar Documents

High-throughput tracking of single yeast cells in a microfluidic imaging matrix

A microfluidic chip for real-time studies of the volume of single cells†

Microfluidic heart on a chip for higher throughput pharmacological studies

Engineering anastomosis between living capillary networks and endothelial cell-lined microfluidic channels

A microfluidic processor for gene expression profiling of single human embryonic stem cells

The single-cell chemostat: an agarose-based, microfluidic device for high-throughput, single-cell studies of bacteria and bacterial communities

Design, Fabrication and Implementation of a Novel Multi Parameter Control Microfluidic Platform for Three-Dimensional Cell Culture and Real-Time Imaging

A microfluidic platform for chemical stimulation and real time analysis of catecholamine secretion from neuroendocrine cells

Methods to array photonic crystal microcavities for high throughput high sensitivity biosensing on a silicon-chip based platform

A high-throughput microfluidic real-time gene expression living cell array †