NDLI: Uptake of Gentamicin by Bullfrog Saccular Hair Cells in vitro

Content Provider	PubMed Central
Author	Steyger, P. S. Peters, S. L. Rehling, J. Hordichok, A. Dai, C. F.
Abstract	Vertebrate sensory hair cells in the inner ear are pharmacologically sensitive to aminoglycoside antibiotics. Although the ototoxicity of aminoglycosides is well known, the route of drug uptake by hair cells and mechanisms of cytotoxicity remain poorly understood. Previously published studies have documented the intracellular distribution of gentamicin using immunocytochemical, electron microscopic, and autoradiographic methods. In this article, we compare the subcellular distribution of fluorescently conjugated gentamicin (gentamicin–Texas Red, GTTR) with immunolabeled gentamicin using confocal or electron microscopy. Gentamicin (detected by postfixation immunocytochemistry) and GTTR were rapidly taken up by hair cells throughout the bullfrog saccular explant in vitro and preferentially in peripheral hair cells. Immunolabeled gentamicin and GTTR were observed at the apical membranes of hair cells, particularly in their hair bundles. GTTR was also identified within a variety of subcellular compartments within hair cells, including lysosomes, mitochondria, Golgi bodies, endoplasmic reticulum, and nuclei, and in similar structures by immunoelectron microscopy. The distributions of GTTR and immunolabeled gentamicin are largely identical and corroborate a variety of published immunocytochemical and autoradiography studies. Thus, GTTR is a valid fluorescent probe with which to investigate the pharmacokinetics and mechanisms of gentamicin accumulation.
Related Links	http://dx.doi.org/10.1007/s10162-003-4002-5
Ending Page	578
Page Count	14
Starting Page	565
File Format	PDF
ISSN	15253961
e-ISSN	14387573
Journal	JARO: Journal of the Association for Research in Otolaryngology
Issue Number	4
Volume Number	4
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-12-01
Access Restriction	Open
Rights Holder	Springer-Verlag
Subject Keyword	Sensory Systems Otorhinolaryngology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Otorhinolaryngology Sensory Systems

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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Uptake of gentamicin by bullfrog saccular hair cells in vitro.

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Uptake of gentamicin by bullfrog saccular hair cells in vitro.

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