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| Content Provider | PubMed Central |
|---|---|
| Author | Armstrong, Philip M. Andreadis, Theodore G. Finan, Shannon L. Shepard, John J. Thomas, Michael C. |
| Copyright Year | 2011 |
| Abstract | Mosquitoes transmit a number of distinct viruses including important human pathogens such as West Nile virus, dengue virus, and chickungunya virus. Many of these viruses have intensified in their endemic ranges and expanded to new territories, necessitating effective surveillance and control programs to respond to these threats. One strategy to monitor virus activity involves collecting large numbers of mosquitoes from endemic sites and testing them for viral infection. In this article, we describe how to handle, process, and screen field-collected mosquitoes for infectious virus by Vero cell culture assay. Mosquitoes are sorted by trap location and species, and grouped into pools containing ≤50 individuals. Pooled specimens are homogenized in buffered saline using a mixer-mill and the aqueous phase is inoculated onto confluent Vero cell cultures (Clone E6). Cell cultures are monitored for cytopathic effect from days 3-7 post-inoculation and any viruses grown in cell culture are identified by the appropriate diagnostic assays. By utilizing this approach, we have isolated 9 different viruses from mosquitoes collected in Connecticut, USA, and among these, 5 are known to cause human disease. Three of these viruses (West Nile virus, Potosi virus, and La Crosse virus) represent new records for North America or the New England region since 1999. The ability to detect a wide diversity of viruses is critical to monitoring both established and newly emerging viruses in the mosquito population. |
| Related Links | http://dx.doi.org/10.3791/2889 |
| Starting Page | 2889 |
| File Format | |
| ISSN | 1940087X |
| e-ISSN | 1940087X |
| Journal | Journal of Visualized Experiments : JoVE |
| Issue Number | 52 |
| Language | English |
| Publisher | MyJove Corporation |
| Publisher Date | 2011-06-01 |
| Access Restriction | Open |
| Rights Holder | MyJove Corporation |
| Subject Keyword | Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Chemical Engineering(all) Neuroscience(all) Medicine(all) Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Neuroscience Immunology and Microbiology Biochemistry, Genetics and Molecular Biology Chemical Engineering |
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