NDLI: Adipose-selective overexpression of ABHD5/CGI-58 does not increase lipolysis or protect against diet-induced obesity

Content Provider	PubMed Central
Author	Caviglia, Jorge M. Betters, Jenna L. Dapito, Dianne-helerie Lord, Caleb C. Sullivan, Sean Chua, Streamson Yin, Terry Anna, Sekowski Mu, Haiyan Shapiro, Lawrence Brown, J. Mark Brasaemle, Dawn L.
Copyright Year	2011
Abstract	Adipose triglyceride lipase (ATGL) catalyzes the first step of triacylglycerol hydrolysis in adipocytes. Abhydrolase domain 5 (ABHD5) increases ATGL activity by an unknown mechanism. Prior studies have suggested that the expression of ABHD5 is limiting for lipolysis in adipocytes, as addition of recombinant ABHD5 increases in vitro TAG hydrolase activity of adipocyte lysates. To test this hypothesis in vivo, we generated transgenic mice that express 6-fold higher ABHD5 in adipose tissue relative to wild-type (WT) mice. In vivo lipolysis increased to a similar extent in ABHD5 transgenic and WT mice following an overnight fast or injection of either a β-adrenergic receptor agonist or lipopolysaccharide. Similarly, basal and β-adrenergic-stimulated lipolysis was comparable in adipocytes isolated from ABHD5 transgenic and WT mice. Although ABHD5 expression was elevated in thioglycolate-elicited macrophages from ABHD5 transgenic mice, Toll-like receptor 4 (TLR4) signaling was comparable in macrophages isolated from ABHD5 transgenic and WT mice. Overexpression of ABHD5 did not prevent the development of obesity in mice fed a high-fat diet, as shown by comparison of body weight, body fat percentage, and adipocyte hypertrophy of ABHD5 transgenic to WT mice. The expression of ABHD5 in mouse adipose tissue is not limiting for either basal or stimulated lipolysis.
Related Links	http://dx.doi.org/10.1194/jlr.m019117
Ending Page	2042
Page Count	11
Starting Page	2032
File Format	PDF
ISSN	00222275
e-ISSN	15397262
Journal	Journal of Lipid Research
Issue Number	11
Volume Number	52
Language	English
Publisher	The American Society for Biochemistry and Molecular Biology
Publisher Date	2011-11-01
Access Restriction	Open
Rights Holder	The American Society for Biochemistry and Molecular Biology
Subject Keyword	Cell Biology Biochemistry Endocrinology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Endocrinology

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Adipose-selective overexpression of ABHD5/CGI-58 does not increase lipolysis or protect against diet-induced obesity

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