NDLI: The contribution of the N- and C-terminal regions of steroid receptors to activation of transcription is both receptor and cell-specific.

Please wait, while we are loading the content...

Year: 2016, Volume: 44

Year: 2015, Volume: 43

Year: 2014, Volume: 42

Year: 2013, Volume: 41

Year: 2012, Volume: 40

Year: 2011, Volume: 39

Year: 2010, Volume: 38

Year: 2009, Volume: 37

Year: 2008, Volume: 37

Year: 2008, Volume: 36

Year: 2007, Volume: 36

Year: 2007, Volume: 35

Year: 2006, Volume: 35

Year: 2006, Volume: 34

Year: 2005, Volume: 33

Year: 2004, Volume: 33

Year: 2004, Volume: 32

Year: 2003, Volume: 31

Year: 2002, Volume: 30

Year: 2001, Volume: 29

Year: 2000, Volume: 28

Year: 1999, Volume: 27

Year: 1998, Volume: 26

Year: 1997, Volume: 25

Year: 1996, Volume: 24

Year: 1995, Volume: 23

Year: 1994, Volume: 22

Year: 1993, Volume: 21

Year: 1992, Volume: 20

Year: 1991, Volume: 19

Year: 1990, Volume: 18

Year: 1989, Volume: 17

Year: 1989, Volume: 17, Issue: Suppl

Year: 1989, Volume: 17, Issue: 24

Year: 1989, Volume: 17, Issue: 23

Year: 1989, Volume: 17, Issue: 22

Year: 1989, Volume: 17, Issue: 21

Year: 1989, Volume: 17, Issue: 20

Year: 1989, Volume: 17, Issue: 19

Year: 1989, Volume: 17, Issue: 18

Year: 1989, Volume: 17, Issue: 17

Year: 1989, Volume: 17, Issue: 16

Year: 1989, Volume: 17, Issue: 15

Year: 1989, Volume: 17, Issue: 14

Year: 1989, Volume: 17, Issue: 13

Year: 1989, Volume: 17, Issue: 12

Year: 1989, Volume: 17, Issue: 11

Year: 1989, Volume: 17, Issue: 10

Year: 1989, Volume: 17, Issue: 9

Year: 1989, Volume: 17, Issue: 8

Year: 1989, Volume: 17, Issue: 7

Solid-phase synthesis of oligoribonucleotides using 9-fluorenylmethoxycarbonyl (Fmoc) for 5'-hydroxyl protection.

Stability and expression of bacterial genes in replicating geminivirus vectors in plants.

Purification and characterization of RepA, a protein involved in the copy number control of plasmid pLS1.

Predictive motifs derived from cytosine methyltransferases.

Detection of single DNA base differences by competitive oligonucleotide priming.

Expression of a mouse replacement histone H3.3 gene with a highly conserved 3' noncoding region during SV40- and polyoma-induced Go to S-phase transition.

Modulations of the in vitro translational efficiencies of Yellow Fever virus mRNAs: interactions between coding and noncoding regions.

Sequence conservation in Alu evolution.

Kinetics of exchangeable protons in Z DNA: a UV resonance Raman study.

Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS).

Direct genomic fluorescent on-line sequencing and analysis using in vitro amplification of DNA.

Occurrence and functional compatibility within Enterobacteriaceae of a tRNA species which inserts selenocysteine into protein.

The GTm6AC sequence is overwound and bent.

Secondary structure analysis of the RepA mRNA leader transcript involved in control of replication of plasmid R1.

The contribution of the N- and C-terminal regions of steroid receptors to activation of transcription is both receptor and cell-specific.

Mutations in the RNA polymerase recognition sequence of the Klebsiella pneumoniae nifH promoter permitting transcriptional activation in the absence of NifA binding to upstream activator sequences.

Three mitochondrial tRNA genes from Arabidopsis thaliana: evidence for the conversion of a tRNAPhe gene into a tRNATyr gene.

Purification and characterization of two serine isoacceptor tRNAs from bovine mitochondria by using a hybridization assay method.

Measurement of the binding of transcription factor Sp1 to a single GC box recognition sequence.

Evidence for nuclear factors involved in recognition of 5' splice sites.

The degree of ultraviolet light damage to DNA containing iododeoxyuridine or bromodeoxyuridine is dependent on the DNA sequence.

Alpha-DNA VIII: thermodynamic parameters of complexes formed between the oligo-alpha-deoxynucleotides: alpha-d(GGAAGG) and alpha-d(CCTTCC) and their complementary oligo-beta-deoxynucleotides: beta-d(CCTTCC) and beta-d(GGAAGG) are different.

DNA recognition element required for PUF-I mediated cell-type-specific transcription of the rat prolactin gene.

The conserved 900 stem/loop region in Escherichia coli 16S ribosomal RNA is not required for protein synthesis.

Analysis of Ori-S sequence of HSV-1: identification of one functional DNA binding domain.

Ventricular myosin light chain 1 is developmentally regulated and does not change in hypertension.

Definition of a second dimeric subfamily of human alpha satellite DNA.

Overlapping redundant septuplets identical with regulatory elements of HIV-1 and SV40.

Construction of a Not I restriction map of the fission yeast Schizosaccharomyces pombe genome.

DNA topology of the ordered chromatin domain 5' to the human c-myc gene.

Glucocorticoid and estrogen regulation of a rat T-kininogen gene.

Nucleotide sequence of the F41 fimbriae subunit gene in Escherichia coli B41.

Nucleotide sequence of a cDNA encoding a larval alpha-globin chain of the amphibian Pleurodeles waltlii.

Nucleotide sequence of cDNA for rat brain and liver cytochrome c oxidase subunit IV.

Nucleotide sequence of the 18S-25S spacer region from rDNA of mung bean.

Sequence of cDNA for a novel light-induced glycine-rich protein.

Nucleotide sequence of the ugpQ gene encoding glycerophosphoryl diester phosphodiesterase of Escherichia coli K-12.

Total coding sequence of profilin cDNA from Mus musculus macrophage.

Nucleotide sequence of the metA gene encoding homoserine trans-succinylase in Escherichia coli.

The nucleotide sequences of nuclear 5S rRNA genes and spacer regions of Petunia hybrida.

Nucleotide sequence of the barley chloroplast psbE, psbF genes and flanking regions.

Nucleotide sequence of the 5.2 kbp barley chloroplast DNA fragment, containing psbB-psbH-petB-petD gene cluster.

Nucleotide sequence of the gene encoding zona pellucida glycoprotein ZP3--the mouse sperm receptor.

Sequence of cDNA for murine Zfy-1, a candidate for Tdy.

The sequence of the rice phytochrome gene.

The nucleotide sequence of rat heparin binding growth factor 1 (HBGF-1).

A calmodulin pseudogene on human chromosome 17.

Human beta-casein: partial cDNA sequence and apparent polymorphism.

Nucleotide sequences of influenza A virus RNA segment 7: a comparison of five isolates.

Structure of an Arabidopsis thaliana cDNA encoding rubisco activase.

The use of filter-bound DNA fragments as templates for radiolabelling by random priming.

Directed mutagenesis using PCR.

Versatile vectors for pulsed expression in eukaryotic cells.

An Apa I polymorphism for the human inter-alpha-trypsin inhibitor heavy chain gene ITIH1 on chromosome 3.

Six RFLPs for human T cell receptor alpha (TCRA) on chromosome 14.

An HaeIII RFLP for the human homeo box-containing gene EN1.

An SstI RFLP for the human homeo box-containing gene EN2.

PvuII and RsaI RFLPs for the human homeo box-containing gene EN2.

An StuI RFLP at the human cholesteryl ester transfer protein (CETP) locus.

A restriction fragment length polymorphism at murine Glud locus co-segregates with Rib-1, Es-10, and Tcra on chromosome 14.

An EcoRI polymorphism of the human von Willebrand factor cDNA (VWF).

An anonymous single-copy clone, p30-1-60, identifies a frequent RFLP on chromosome 3p [HGM9 no. D3S86].

A polymorphic locus at Xq27-28 detected by the probe U6.2 [DXS304].

A second Xenopus immunoglobulin heavy chain constant region isotype gene

Year: 1989, Volume: 17, Issue: 6

Year: 1989, Volume: 17, Issue: 5

Year: 1989, Volume: 17, Issue: 4

Year: 1989, Volume: 17, Issue: 3

Year: 1989, Volume: 17, Issue: 2

Year: 1989, Volume: 17, Issue: 1

Year: 1988, Volume: 16

Year: 1987, Volume: 15

Year: 1986, Volume: 14

Year: 1985, Volume: 13

Year: 1984, Volume: 12

Year: 1983, Volume: 11

Year: 1982, Volume: 10

Year: 1981, Volume: 9

Year: 1980, Volume: 8

Year: 1979, Volume: 7

Year: 1979, Volume: 6

Year: 1978, Volume: 5

Year: 1977, Volume: 4

Year: 1976, Volume: 3

Year: 1975, Volume: 2

Year: 1974, Volume: 1

The contribution of the N- and C-terminal regions of steroid receptors to activation of transcription is both receptor and cell-specific.

Content Provider	PubMed Central
Author	Bocquel, M. T. Stricker, C. Chambon, P. Gronemeyer, H. Kumar, V.
Abstract	Normalized dose response-curves for transcriptional activation of reporter genes were obtained by co-transfecting them with increasing amounts of wild-type (wt) progesterone (PR), glucocorticoid (GR) and oestrogen (ER) expression vectors. Marked differences in both shape and magnitude of the stimulation were observed depending on whether HeLa or CV1 cells were transfected. In HeLa cells the transcriptional stimulation from a reporter gene containing the hormone responsive element (RE) present in the mouse mammary tumour virus (MMTV) long terminal repeat (LTR) increased as increasing amounts (from 0.05 to 7.5 micrograms) of PR expression vector were transfected, whereas no such increase was observed in CV1 cells above 1 microgram of the same vector. In contrast, a PR mutant lacking the hormone binding domain (HBD, region E), exhibited increasing constitutive activity with increasing amounts of PR expression vector, such that in CV1 cells, but not in HeLa cells, similar activities were measured for the mutant and wt PR when 5 micrograms expression vectors were transfected. Western blot analyses indicated that the differences between the two cell lines were not due to differences in the amount of receptor proteins. Using the same MMTV LTR-based reporter gene, cell-specific differences were also detected between the dose-response curves obtained for the human GR and a mutant which lacks the HBD. A PR mutant in which the N-terminal A/B region was deleted exhibited no (CV1 cells) or less than 5% (HeLa cells) of the wt-activity, whereas the corresponding GR mutant stimulated efficiently transcription in both cell lines. Identical studies with the wt human ER or a mutant truncated for the N-terminal A/B region resulted in bell-shaped dose-response curves in both HeLa and CV1 cells, whereas an ER mutant lacking the HBD was weakly active in either cell line. These data demonstrate cell- and receptor-specificity for the transcriptional activation functions present in the A/B region and the HBD of various steroid receptors and suggest that limiting factors mediate their action. The present study also emphasizes the need of establishing dose-response curves to correctly assess the relative contribution of the different regions of steroid hormone receptors in activation of transcription.
Starting Page	2581
File Format	PDF
ISSN	13624962
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	7
Volume Number	17
Language	English
Publisher Date	1989-04-11
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in