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| Content Provider | PubMed Central |
|---|---|
| Author | Pasero, Philippe Duncker, Bernard P. Schwob, Etienne Gasser, Susan M. |
| Copyright Year | 1999 |
| Abstract | Using a reconstituted DNA replication assay from yeast, we demonstrate that two kinase complexes are essential for the promotion of replication in vitro. An active Clb/Cdc28 kinase complex, or its vertebrate equivalent, is required in trans to stimulate initiation in G1-phase nuclei, whereas the Dbf4/Cdc7 kinase complex must be provided by the template nuclei themselves. The regulatory subunit of Cdc7p, Dbf4p, accumulates during late G1 phase, becomes chromatin associated prior to Clb/Cdc28 activation, and assumes a punctate pattern of localization that is similar to, and dependent on, the origin recognition complex (ORC). The association of Dbf4p with a detergent-insoluble chromatin fraction in G1-phase nuclei requires ORC but not Cdc6p or Clb/Cdc28 kinase activity, and correlates with competence for initiation. We propose a model in which Dbf4p targets Cdc7p to the prereplication complex prior to the G1/S transition, by a pathway parallel to, but independent of, the Cdc6p-dependent recruitment of MCMs. |
| Starting Page | 2159 |
| File Format | |
| ISSN | 08909369 |
| Journal | Genes & Development |
| Issue Number | 16 |
| Volume Number | 13 |
| Language | English |
| Publisher | Cold Spring Harbor Laboratory Press |
| Publisher Date | 1999-08-15 |
| Access Restriction | Open |
| Rights Holder | Cold Spring Harbor Laboratory Press |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Developmental Biology |
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