NDLI: Novel Apparatus and Method for Drug Reinforcement

Content Provider	PubMed Central
Author	Feduccia, Allison A. Duvauchelle, Christine L.
Copyright Year	2010
Abstract	Animal models of reinforcement have proven to be useful for understanding the neurobiological mechanisms underlying drug addiction. Operant drug self-administration and conditioned place preference (CPP) procedures are expansively used in animal research to model various components of drug reinforcement, consumption, and addiction in humans. For this study, we used a novel approach to studying drug reinforcement in rats by combining traditional CPP and self-administration methodologies. We assembled an apparatus using two Med Associate operant chambers, sensory stimuli, and a Plexiglas-constructed neutral zone. These modifications allowed our experiments to encompass motivational aspects of drug intake through self-administration and drug-free assessment of drug/cue conditioning strength with the CPP test. In our experiments, rats self-administered cocaine (0.75 mg/kg/inj, i.v.) during either four (e.g., the "short-term") or eight (e.g., the "long-term") alternating-day sessions in an operant environment containing distinctive sensory cues (e.g., olfactory and visual). On the alternate days, in the other (differently-cued) operant environment, saline was available for self-infusion (0.1 ml, i.v.). Twenty-four hours after the last self-administration/cue-pairing session, a CPP test was conducted. Consistent with typical CPP findings, there was a significant preference for the chamber associated with cocaine self-administration. In addition, in animals undergoing the long-term experiment, a significant positive correlation between CPP magnitude and the number of cocaine-reinforced lever responses. In conclusion, this apparatus and approach is time and cost effective, can be used to examine a wide array of topics pertaining to drug abuse, and provides more flexibility in experimental design than CPP or self-administration methods alone.
Related Links	http://dx.doi.org/10.3791/1998
Starting Page	1998
File Format	PDF
ISSN	1940087X
e-ISSN	1940087X
Journal	Journal of Visualized Experiments : JoVE
Issue Number	42
Language	English
Publisher	MyJove Corporation
Publisher Date	2010-08-01
Access Restriction	Open
Rights Holder	MyJove Corporation
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Chemical Engineering(all) Neuroscience(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Biochemistry, Genetics and Molecular Biology Chemical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Method for Novel Anti-Cancer Drug Development using Tumor Explants of Surgical Specimens

A Novel Vertebral Stabilization Method for Producing Contusive Spinal Cord Injury

Electrospinning Fundamentals: Optimizing Solution and Apparatus Parameters

High-throughput Detection Method for Influenza Virus

Particle Agglutination Method for Poliovirus Identification

Pyrosequencing: A Simple Method for Accurate Genotyping

Functional Imaging with Reinforcement, Eyetracking, and Physiological Monitoring

Method for Whole Mount Antibody Staining in Chick

Yeast Colony Embedding Method

Novel Apparatus and Method for Drug Reinforcement

Similar Documents

Method for Novel Anti-Cancer Drug Development using Tumor Explants of Surgical Specimens

A Novel Vertebral Stabilization Method for Producing Contusive Spinal Cord Injury

Electrospinning Fundamentals: Optimizing Solution and Apparatus Parameters

High-throughput Detection Method for Influenza Virus

Particle Agglutination Method for Poliovirus Identification

Pyrosequencing: A Simple Method for Accurate Genotyping

Functional Imaging with Reinforcement, Eyetracking, and Physiological Monitoring

Method for Whole Mount Antibody Staining in Chick

Yeast Colony Embedding Method

Novel Apparatus and Method for Drug Reinforcement