NDLI: Laboratory Adaptation of Bordetella pertussis Is Associated with the Loss of Type Three Secretion System Functionality ▿

Content Provider	PubMed Central
Author	Gaillard, M. E. Bottero, D. Castuma, C. E. Basile, L. A. Hozbor, D.
Editor	Bliska, J. B.
Copyright Year	2011
Abstract	Although Bordetella pertussis contains and transcribes loci encoding type III secretion system (TTSS) homologues, expression of TTSS-associated proteins has been reported only for non-laboratory-adapted Irish clinical isolates. Here we confirm such a result for clinical isolates obtained from patients treated in Argentinean hospitals. Moreover, we demonstrate that the expression of TTSS-associated proteins is independent both of the year in which the isolate was obtained and of the types of polymorphic alleles for other virulence factors but is dependent on environmental growth conditions. Interestingly, we observed that TTSS-associated protein expression is lost after successive in vitro passages but becomes operative again when bacteria come into contact with the host. This in vivo activation of TTSS expression was observed not only for clinical isolates previously adapted to the laboratory after successive in vitro passages but also for vaccine strains that did not express the system in vitro. The reversibility of TTSS expression, demonstrated by its switching off-on when the bacterium comes into contact with the host, appears to be an adaptive response of this pathogen.
Related Links	http://dx.doi.org/10.1128/iai.00136-11
Ending Page	3682
Page Count	6
Starting Page	3677
File Format	PDF
ISSN	00199567
e-ISSN	10985522
Journal	Infection and Immunity
Issue Number	9
Volume Number	79
Language	English
Publisher	American Society for Microbiology
Publisher Date	2011-09-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Immunology Microbiology Parasitology Infectious Diseases Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology Immunology Microbiology

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Laboratory adaptation of Bordetella pertussis is associated with the loss of type three secretion system functionality.

Reduced Glutathione Is Required for Pertussis Toxin Secretion by Bordetella pertussis

DsbA and DsbC Are Required for Secretion of Pertussis Toxin by Bordetella pertussis

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Laboratory Adaptation of Bordetella pertussis Is Associated with the Loss of Type Three Secretion System Functionality ▿

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Laboratory adaptation of Bordetella pertussis is associated with the loss of type three secretion system functionality.

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