NDLI: Imaging Long-Term Fate of Intramyocardially Implanted Mesenchymal Stem Cells in a Porcine Myocardial Infarction Model

Content Provider	PubMed Central
Author	Perin, Emerson C. Tian, Mei Marini, Frank C. Silva, Guilherme V. Zheng, Yi Fred, Baimbridge Quan, Xin Fernandes, Marlos R. Amir, Gahremanpour Young, Daniel Paolillo, Vincenzo Mukhopadhyay, Uday Borne, Agatha T. Rajesh, Uthamanthil Brammer, David Jackson, James Decker, William K. Najjar, Amer M. Thomas, Michael W. Volgin, Andrei Rabinovich, Brian Suren, Soghomonyan Jeong, Hwan-jeong Rios, Jesse M. Steiner, David Robinson, Simon Mawlawi, Osama Pan, Tinsu Stafford, Jason Kundra, Vikas Li, Chun Alauddin, Mian M. Willerson, James T. Elizabeth, Shpall Gelovani, Juri G.
Editor	Gambhir, Sanjiv Sam
Copyright Year	2011
Abstract	The long-term fate of stem cells after intramyocardial delivery is unknown. We used noninvasive, repetitive PET/CT imaging with [18F]FEAU to monitor the long-term (up to 5 months) spatial-temporal dynamics of MSCs retrovirally transduced with the sr39HSV1-tk gene (sr39HSV1-tk-MSC) and implanted intramyocardially in pigs with induced acute myocardial infarction. Repetitive [18F]FEAU PET/CT revealed a biphasic pattern of sr39HSV1-tk-MSC dynamics; cell proliferation peaked at 33–35 days after injection, in periinfarct regions and the major cardiac lymphatic vessels and lymph nodes. The sr39HSV1-tk-MSC–associated [18F]FEAU signals gradually decreased thereafter. Cardiac lymphography studies using PG-Gd-NIRF813 contrast for MRI and near-infrared fluorescence imaging showed rapid clearance of the contrast from the site of intramyocardial injection through the subepicardial lymphatic network into the lymphatic vessels and periaortic lymph nodes. Immunohistochemical analysis of cardiac tissue obtained at 35 and 150 days demonstrated several types of sr39HSV1-tk expressing cells, including fibro-myoblasts, lymphovascular cells, and microvascular and arterial endothelium. In summary, this study demonstrated the feasibility and sensitivity of [18F]FEAU PET/CT imaging for long-term, in-vivo monitoring (up to 5 months) of the fate of intramyocardially injected sr39HSV1-tk-MSC cells. Intramyocardially transplanted MSCs appear to integrate into the lymphatic endothelium and may help improve myocardial lymphatic system function after MI.
Related Links	http://dx.doi.org/10.1371/journal.pone.0022949
Starting Page	22949
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	9
Volume Number	6
Language	English
Publisher	Public Library of Science
Publisher Date	2011-09-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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