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| Content Provider | PubMed Central |
|---|---|
| Author | Ford, Lance P. Watson, Janice Keene, Jack D. Wilusz, Jeffrey |
| Copyright Year | 1999 |
| Abstract | We have developed an in vitro mRNA stability system using HeLa cell cytoplasmic S100 extracts and exogenous polyadenylated RNA substrates that reproduces regulated aspects of mRNA decay. The addition of cold poly(A) competitor RNA activated both a sequence-specific deadenylase activity in the extracts as well as a potent, ATP-dependent ribonucleolytic activity. The rates of both deadenylation and degradation were up-regulated by the presence of a variety of AU-rich elements in the body of substrate RNAs. Competition analyses demonstrated that trans-acting factors were required for RNA destabilization by AU-rich elements. The ∼30-kD ELAV protein HuR specifically bound to RNAs containing an AU-rich element derived from the TNF-α mRNA in the in vitro system. Interaction of HuR with AU-rich elements, however, was not associated with RNA destabilization. Interestingly, recombinant ELAV proteins specifically stabilized deadenylated intermediates generated from the turnover of AU-rich element-containing substrate RNAs. These data suggest that mammalian ELAV proteins play a role in regulating mRNA stability by influencing the access of degradative enzymes to RNA substrates. |
| Starting Page | 188 |
| File Format | |
| ISSN | 08909369 |
| Journal | Genes & Development |
| Issue Number | 2 |
| Volume Number | 13 |
| Language | English |
| Publisher | Cold Spring Harbor Laboratory Press |
| Publisher Date | 1999-01-15 |
| Access Restriction | Open |
| Rights Holder | Cold Spring Harbor Laboratory Press |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Developmental Biology |
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