NDLI: Biotin Transport and Accumulation by Cells of Lactobacillus plantarum II. Kinetics of the System

Content Provider	PubMed Central
Author	Waller, James R. Lichstein, Herman C.
Abstract	Waller, James R. (University of Cincinnati, Cincinnati, Ohio), and Herman C. Lichstein. Biotin transport and accumulation by cells of Lactobacillus plantarum. II. Kinetics of the system. J. Bacteriol. 90:853–856. 1965.—Bound biotin-saturated cells of Lactobacillus plantarum accumulated free biotin by a time-dependent process exhibiting substrate saturation phenomena in the presence and absence of glucose. Apparent K m and V max values determined in the presence and absence of glucose, respectively, from Lineweaver-Burk plots were found to be 31.5 and 7.72 mμm (K m) and 9.72 and 3.26 μμmoles per mg per min (V max). Free biotin transport per se appeared to be an energy-independent, mediated process, whereas the accumulation of large intracellular vitamin concentrations was energy-dependent. Internal free biotin was quantitatively converted to bound biotin. The rate and extent of bound biotin formation was slower than free biotin uptake, and dependent upon intracellular free biotin levels up to a saturating concentration.
Starting Page	853
File Format	PDF
ISSN	10985530
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	4
Volume Number	90
Language	English
Publisher Date	1965-10-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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Biotin Transport and Accumulation by Cells of Lactobacillus plantarum II. Kinetics of the System