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| Content Provider | PubMed Central |
|---|---|
| Author | Isikman, Serhan O. Bishara, Waheb Sikora, Uzair Yaglidere, Oguzhan Yeah, John Ozcan, Aydogan |
| Copyright Year | 2011 |
| Abstract | We present a field-portable lensfree tomographic microscope, which can achieve sectional imaging of a large volume (~20 mm3) on a chip with an axial resolution of <7 μm. In this compact tomographic imaging platform (weighing only ~110 grams), 24 light-emitting diodes (LEDs) that are each butt-coupled to a fibre-optic waveguide are controlled through a cost-effective micro-processor to sequentially illuminate the sample from different angles to record lensfree holograms of the sample that is placed on the top of a digital sensor array. In order to generate pixel super-resolved (SR) lensfree holograms and hence digitally improve the achievable lateral resolution, multiple sub-pixel shifted holograms are recorded at each illumination angle by electromagnetically actuating the fibre-optic waveguides using compact coils and magnets. These SR projection holograms obtained over an angular range of ~50° are rapidly reconstructed to yield projection images of the sample, which can then be back-projected to compute tomograms of the objects on the sensor-chip. The performance of this compact and light-weight lensfree tomographic microscope is validated by imaging micro-beads of different dimensions as well as a Hymenolepis nana egg, which is an infectious parasitic flatworm. Achieving a decent three-dimensional spatial resolution, this field-portable on-chip optical tomographic microscope might provide a useful toolset for telemedicine and high-throughput imaging applications in resource-poor settings. |
| Related Links | http://dx.doi.org/10.1039/c1lc20127a |
| Starting Page | 2222 |
| File Format | |
| ISSN | 14730197 |
| e-ISSN | 14730189 |
| Journal | Lab on a chip |
| Issue Number | 13 |
| Volume Number | 11 |
| Language | English |
| Publisher Date | 2011-01-01 |
| Access Restriction | Open |
| Subject Keyword | Biochemistry Bioengineering Chemistry(all) Biomedical Engineering Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering |
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