NDLI: Engineered Polymer-Media Interfaces for the Long-term Self-renewal of Human Embryonic Stem Cells

Content Provider	PubMed Central
Author	Irwin, Elizabeth F. Gupta, Rohini Dashti, Derek C. Healy, Kevin E.
Copyright Year	2011
Abstract	We have developed a synthetic polymer interface for the long-term self-renewal of human embryonic stem cells (hESCs) in defined media. We successfully cultured hESCs on hydrogel interfaces of aminopropylmethacrylamide (APMAAm) for over 20 passages in chemically-defined mTeSR™ 1 media and demonstrated pluripotency of multiple hESC lines with immunostaining and quantitative RT-PCR studies. Results for hESC proliferation and pluripotency markers were both qualitatively and quantitatively similar to cells cultured on Matrigel™ -coated substrates. Mechanistically, it was resolved that bovine serum albumin (BSA) in the mTeSR™ 1 media was critical for cell adhesion on APMAAm hydrogel interfaces. This study uniquely identified a robust long-term culture surface for the self-renewal of hESCs without the use of biologic coatings (e.g., peptides, proteins, or Matrigel™) in completely chemically-defined media that employed practical culturing techniques amenable to clinical-scale cell expansion.
Related Links	http://dx.doi.org/10.1016/j.biomaterials.2011.05.058
Ending Page	6919
Page Count	8
Starting Page	6912
File Format	PDF
ISSN	01429612
e-ISSN	18785905
Journal	Biomaterials
Issue Number	29
Volume Number	32
Language	English
Publisher Date	2011-10-01
Access Restriction	Open
Subject Keyword	Biophysics Mechanics of Materials Bioengineering Biomaterials Ceramics and Composites Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ceramics and Composites Nanoscience and Nanotechnology Mechanics of Materials Biomaterials Biophysics Bioengineering

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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