NDLI: A deoxyribozyme that harnesses light to repair thymine dimers in DNA

Content Provider	PubMed Central
Author	Chinnapen, Daniel J. F. Sen, Dipankar
Copyright Year	2004
Abstract	In vitro selection was used to investigate whether nucleic acid enzymes are capable of catalyzing photochemical reactions. The reaction chosen was photoreactivation of thymine cyclobutane dimers in DNA by using serotonin as cofactor and light of wavelengths longer than the absorption spectrum of DNA. Curiously, the dominant single-stranded DNA sequence selected, UV1A, was found to repair its internal thymine dimer substrate efficiently even in the absence of serotonin or any other cofactor. UV1C, a 42-nucleotide fragment of UV1A, repaired the thymine dimer substrate in trans (k cat/k uncat = 2.5 × 104), showing optimal activity with 305 nm light and thus resembling naturally occurring photolyase enzymes. Mechanistic investigation of UV1C indicated that its catalytic role likely exceeded the mere positioning of the substrate in a conformation favorable for photoreactivation. A higher-order structure, likely a quadruplex, formed by specific guanine bases within the deoxyribozyme, was implicated as serving as a light-harvesting antenna, with photoreactivation of the thymine dimer proceeding possibly via electron donation from an excited guanine base. In a primordial “RNA world,” self-replicating nucleic acid populations may have been vulnerable to deactivation via UV light-mediated pyrimidine dimer formation. Photolyase nucleic acid enzymes such as the one described here could thus have played a role in preserving the integrity of such an RNA world.
Related Links	http://dx.doi.org/10.1073/pnas.0305943101
Starting Page	65
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	1
Volume Number	101
Language	English
Publisher	National Academy of Sciences
Publisher Date	2004-01-06
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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EVIDENCE THAT ULTRAVIOLET-INDUCED THYMINE DIMERS IN DNA CAUSE BIOLOGICAL DAMAGE

RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12

Thymine dimers bend DNA.

ENZYMATIC REPAIR OF DNA, I. PURIFICATION OF TWO ENZYMES INVOLVED IN THE EXCISION OF THYMINE DIMERS FROM ULTRAVIOLET-IRRADIATED DNA *

Mechanism of nucleotide incorporation opposite a thymine-thymine dimer by yeast DNA polymerase eta.

ENZYMATIC REPAIR OF DNA, I. PURIFICATION OF TWO ENZYMES INVOLVED IN THE EXCISION OF THYMINE DIMERS FROM ULTRAVIOLET-IRRADIATED DNA

Repair by human cell extracts of single (6-4) and cyclobutane thymine-thymine photoproducts in DNA.

THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM

Direct observation of thymine dimer repair in DNA by photolyase.

A deoxyribozyme that harnesses light to repair thymine dimers in DNA

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