NDLI: A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants

Content Provider	PubMed Central
Author	Kong, Ling-jie Orozco, Beverly M. Roe, Judith L. Nagar, Steve Ou, Sharon Feiler, Heidi S. Durfee, Tim Miller, Ann B. Wilhelm, Gruissem Robertson, Dominique Linda, Hanley-bowdoin
Copyright Year	2000
Abstract	Geminiviruses replicate in nuclei of mature plant cells after inducing the accumulation of host DNA replication machinery. Earlier studies showed that the viral replication factor, AL1, is sufficient for host induction and interacts with the cell cycle regulator, retinoblastoma (pRb). Unlike other DNA virus proteins, AL1 does not contain the pRb binding consensus, LXCXE, and interacts with plant pRb homo logues (pRBR) through a novel amino acid sequence. We mapped the pRBR binding domain of AL1 between amino acids 101 and 180 and identified two mutants that are differentially impacted for AL1–pRBR interactions. Plants infected with the E-N140 mutant, which is wild-type for pRBR binding, developed wild-type symptoms and accumulated viral DNA and AL1 protein in epidermal, mesophyll and vascular cells of mature leaves. Plants inoculated with the KEE146 mutant, which retains 16% pRBR binding activity, only developed chlorosis along the veins, and viral DNA, AL1 protein and the host DNA synthesis factor, proliferating cell nuclear antigen, were localized to vascular tissue. These results established the importance of AL1–pRBR interactions during geminivirus infection of plants.
Related Links	http://dx.doi.org/10.1093/emboj/19.13.3485
Starting Page	3485
File Format	PDF
ISSN	14602075
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	13
Volume Number	19
Language	English
Publisher	Oxford University Press
Publisher Date	2000-07-03
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

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A Novel Motif in Geminivirus Replication Proteins Interacts with the Plant Retinoblastoma-Related Protein

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