NDLI: Chlamydia trachomatis secretion of hypothetical protein CT622 into host cell cytoplasm via a secretion pathway that can be inhibited by the type III secretion system inhibitor compound 1

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Chlamydia trachomatis secretion of hypothetical protein CT622 into host cell cytoplasm via a secretion pathway that can be inhibited by the type III secretion system inhibitor compound 1

Content Provider	PubMed Central
Author	Gong, Siqi Lei, Lei Chang, Xiaotong Belland, Robert Zhong, Guangming
Copyright Year	2011
Abstract	Using antibodies raised with C. trachomatis fusion proteins, we localized a hypothetical protein encoded by the ORF ct622 in the cytoplasm of C. trachomatis-infected mammalian cells. The detection was specific since the antibody labelling of CT622 protein was removed by preabsorption with CT622 but not other fusion proteins. We similarly confirmed that CT621, a known secretion protein encoded by a hypothetical ORF downstream of ct622, was secreted into host cell cytosol. Proteins CT622 and CT621 displayed a similar secretion pattern, with both intra-inclusion and host cell cytosol localization, that was distinct from that of CPAF (chlamydial protease/proteasome-like activity factor). However, the expression and secretion kinetics differed significantly between CT622 and CT621: CT622 mRNA was detected at 2 h, protein at 6 h and secretion of protein into host cell cytoplasm at 36 h post-infection, while CT621 mRNA was detected at 8 h, protein at 16 h and secretion at 24 h. The secretion of both CT622 and CT621 was blocked by N′-(3,5-dibromo-2-hydroxybenzylidene)-4-nitrobenzohydrazide (compound 1), an inhibitor known to target the type III secretion system of bacteria. These results suggest that CT621 and CT622 may fulfil different functions during chlamydial intracellular growth. Further characterization of these proteins may generate important information for understanding chlamydial pathogenesis.
Related Links	http://dx.doi.org/10.1099/mic.0.047746-0
Ending Page	1144
Page Count	11
Starting Page	1134
File Format	PDF
ISSN	13500872
e-ISSN	14652080
Journal	Microbiology
Issue Number	Pt 4
Volume Number	157
Language	English
Publisher	Society for General Microbiology
Publisher Date	2011-04-01
Access Restriction	Open
Rights Holder	Society for General Microbiology
Subject Keyword	Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Microbiology

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