NDLI: Quantitative Time-Lapse Fluorescence Microscopy in Single Cells

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Quantitative Time-Lapse Fluorescence Microscopy in Single Cells

Content Provider	PubMed Central
Author	Muzzey, Dale Oudenaarden, Alexander Van
Abstract	The cloning of GFP 15 years ago revolutionized cell biology by permitting visualization of a wide range of molecular mechanisms within living cells. Though initially used to make largely qualitative assessments of protein levels and localizations, fluorescence microscopy has since evolved to become highly quantitative and high-throughput. Computational image analysis has catalyzed this evolution, enabling rapid and automated processing of large datasets. Here we review studies that combine time-lapse fluorescence microscopy and automated image analysis to investigate dynamic events at the single-cell level. We highlight examples where single-cell analysis provides unique mechanistic insights into cellular processes that cannot be otherwise resolved in bulk assays. Additionally, we discuss studies where quantitative microscopy facilitates the assembly of detailed 4D lineages in developing organisms. Finally, we describe recent advances in imaging technology, focusing especially on platforms that allow the simultaneous perturbation and quantitative monitoring of biological systems.
Related Links	http://dx.doi.org/10.1146/annurev.cellbio.042308.113408
Ending Page	327
Page Count	27
Starting Page	301
File Format	PDF
ISSN	10810706
e-ISSN	15308995
Journal	Annual review of cell and developmental biology
Volume Number	25
Language	English
Publisher Date	2009-11-01
Access Restriction	Open
Subject Keyword	Developmental Biology Cell Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology

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