NDLI: Bi-specific Aptamers Mediating Tumor Cell Lysis

Content Provider	PubMed Central
Author	Boltz, Achim Piater, Birgit Lars, Toleikis Guenther, Ralf Kolmar, Harald Hock, Bjoern
Copyright Year	2011
Abstract	Antibody-dependent cellular cytotoxicity plays a pivotal role in antibody-based tumor therapies and is based on the recruitment of natural killer cells to antibody-bound tumor cells via binding of the Fcγ receptor III (CD16). Here we describe the generation of chimeric DNA aptamers that simultaneously bind to CD16α and c-Met, a receptor that is overexpressed in many tumors. By application of the systematic evolution of ligands by exponential enrichment (SELEX) method, CD16α specific DNA aptamers were isolated that bound with high specificity and affinity (91 pm–195 nm) to their respective recombinant and cellularly expressed target proteins. Two optimized CD16α specific aptamers were coupled to each of two c-Met specific aptamers using different linkers. Bi-specific aptamers retained suitable binding properties and displayed simultaneous binding to both antigens. Moreover, they mediated cellular cytotoxicity dependent on aptamer and effector cell concentration. Displacement of a bi-specific aptamer from CD16α by competing antibody 3G8 reduced cytotoxicity and confirmed the proposed mode of action. These results represent the first gain of a tumor-effective function of two distinct oligonucleotides by linkage into a bi-specific aptamer mediating cellular cytotoxicity.
Related Links	http://dx.doi.org/10.1074/jbc.m111.238261
Ending Page	21905
Page Count	10
Starting Page	21896
File Format	PDF
ISSN	00219258
e-ISSN	1083351X
Journal	The Journal of Biological Chemistry
Issue Number	24
Volume Number	286
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2011-06-17
Access Restriction	Open
Rights Holder	American Society for Biochemistry and Molecular Biology
Subject Keyword	Cell Biology Biochemistry Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Bi-specific Aptamers Mediating Tumor Cell Lysis

Complement-Mediated Selective Tumor Cell Lysis Enabled by Bi-Functional RNA Aptamers

Identification of Epithelial Ovarian Tumor-Specific Aptamers

Cell-Specific Aptamers as Emerging Therapeutics

Targeting 4-1BB Costimulation to Disseminated Tumor Lesions With Bi-specific Oligonucleotide Aptamers

Bi-specific MHC Heterodimers for Characterization of Cross-reactive T Cells*

Cell-Specific Aptamers for Targeted Therapies

Specific Oxidized Phospholipids Inhibit Scavenger Receptor BI-mediated Selective Uptake of Cholesteryl Esters*

Redirecting NK cells mediated tumor cell lysis by a new recombinant bifunctional protein

Bi-specific Aptamers Mediating Tumor Cell Lysis

Similar Documents

Bi-specific Aptamers Mediating Tumor Cell Lysis

Complement-Mediated Selective Tumor Cell Lysis Enabled by Bi-Functional RNA Aptamers

Identification of Epithelial Ovarian Tumor-Specific Aptamers

Cell-Specific Aptamers as Emerging Therapeutics

Targeting 4-1BB Costimulation to Disseminated Tumor Lesions With Bi-specific Oligonucleotide Aptamers

Bi-specific MHC Heterodimers for Characterization of Cross-reactive T Cells*

Cell-Specific Aptamers for Targeted Therapies

Specific Oxidized Phospholipids Inhibit Scavenger Receptor BI-mediated Selective Uptake of Cholesteryl Esters*

Redirecting NK cells mediated tumor cell lysis by a new recombinant bifunctional protein

Bi-specific Aptamers Mediating Tumor Cell Lysis