NDLI: Quantitative analysis of fitness and genetic interactions in yeast on a genome scale

Content Provider	PubMed Central
Author	Baryshnikova, Anastasia Costanzo, Michael Kim, Yungil Ding, Huiming Koh, Judice Toufighi, Kiana Youn, Ji-young Ou, Jiongwen Luis, Bryan-joseph San Bandyopadhyay, Sunayan Hibbs, Matthew Hess, David Gingras, Anne-claude Bader, Gary D. Troyanskaya, Olga G. Brown, Grant W. Andrews, Brenda Boone, Charles Myers, Chad L.
Copyright Year	2010
Abstract	Global quantitative analysis of genetic interactions is a powerful approach for deciphering the roles of genes and mapping functional relationships among pathways. Using colony size as a proxy for fitness, we developed a method for measuring fitness-based genetic interactions from high-density arrays of yeast double mutants generated by synthetic genetic array (SGA) analysis. We identified several experimental sources of systematic variation and developed normalization strategies to obtain accurate single- and double-mutant fitness measurements, which rival the accuracy of other high-resolution studies. We applied the SGA score to examine the relationship between physical and genetic interaction networks, and we found that positive genetic interactions connect across functionally distinct protein complexes revealing a network of genetic suppression among loss-of-function alleles.
Related Links	http://dx.doi.org/10.1038/nmeth.1534
Ending Page	1024
Page Count	8
Starting Page	1017
File Format	PDF
ISSN	15487091
e-ISSN	15487105
Journal	Nature methods
Issue Number	12
Volume Number	7
Language	English
Publisher Date	2010-11-01
Access Restriction	Open
Subject Keyword	Biotechnology Cell Biology Biochemistry Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology Biotechnology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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