NDLI: Orientation and Polarity in Collectively Migrating Cell Structures: Statics and Dynamics

Content Provider	PubMed Central
Author	Reffay, M. Petitjean, L. Coscoy, S. Grasland-mongrain, E. Amblard, F. Buguin, A. Silberzan, P.
Copyright Year	2011
Abstract	Collective cell migration is often characterized by the spontaneous onset of multicellular protrusions (known as fingers) led by a single leader cell. Working with epithelial Madin-Darby canine kidney monolayers we show that cells within the fingers, as compared with the epithelium, are well oriented and polarized along the main finger direction, which suggests that these cells actively migrate. The cell orientation and polarity decrease continuously from the tip toward the epithelium over a penetration distance of typically two finger lengths. Furthermore, laser photoablation experiments at various locations along these fingers demonstrate that the cells in the fingers are submitted to a tensile stress whose value is larger close to the tip. From a dynamical point of view, cells entering a finger gradually polarize on timescales that depend upon their particular initial position. Selective laser nanosurgery of the leader lamellipodium shows not only that these structures need a leader to progress, but that this leader itself is the consequence of a prior self-organization of the cells forming the finger. These results highlight the complex interplay between the collective orientation within the fingers and the mechanical action of the leader.
Related Links	http://dx.doi.org/10.1016/j.bpj.2011.04.047
Ending Page	2575
Page Count	10
Starting Page	2566
File Format	PDF
ISSN	00063495
e-ISSN	15420086
Journal	Biophysical Journal
Issue Number	11
Volume Number	100
Language	English
Publisher	The Biophysical Society
Publisher Date	2011-06-01
Access Restriction	Open
Rights Holder	The Biophysical Society
Subject Keyword	Biophysics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics

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