NDLI: Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells: Tips and Tricks

Content Provider	PubMed Central
Author	Solchaga, Luis A. Penick, Kitsie J. Welter, Jean F.
Copyright Year	2011
Abstract	It is well known that adult cartilage lacks the ability to repair itself; this makes articular cartilage a very attractive target for tissue engineering. The majority of articular cartilage repair models attempt to deliver or recruit reparative cells to the site of injury. A number of efforts are directed to the characterization of progenitor cells and the understanding of the mechanisms involved in their chondrogenic differentiation. Our laboratory has focused on cartilage repair using mesenchymal stem cells and studied their differentiation into cartilage. Mesenchymal stem cells are attractive candidates for cartilage repair due to their osteogenic and chondrogenic potential, ease of harvest, and ease of expansion in culture. However, the need for chondrogenic differentiation is superposed on other technical issues associated with cartilage repair; this adds a level of complexity over using mature chondrocytes. This chapter will focus on the methods involved in the isolation and expansion of human mesenchymal stem cells, their differentiation along the chondrogenic lineage, and the qualitative and quantitative assessment of chondrogenic differentiation.
Related Links	http://dx.doi.org/10.1007/978-1-60761-999-4_20
Ending Page	278
Page Count	26
Starting Page	253
File Format	PDF
ISSN	10643745
e-ISSN	19406029
Journal	Methods in molecular biology (Clifton, N.J.)
Volume Number	698
Language	English
Publisher Date	2011-01-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Molecular Biology

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