NDLI: Sequence and Generation of Mature Ribosomal RNA Transcripts in Dictyostelium discoideum

Content Provider	PubMed Central
Author	Boesler, Carsten Kruse, Janis Söderbom, Fredrik Hammann, Christian
Copyright Year	2011
Abstract	The amoeba Dictyostelium discoideum is a well established model organism for studying numerous aspects of cellular and developmental functions. Its ribosomal RNA (rRNA) is encoded in an extrachromosomal palindrome that exists in ∼100 copies in the cell. In this study, we have set out to investigate the sequence of the expressed rRNA. For this, we have ligated the rRNA ends and performed RT-PCR on these circular RNAs. Sequencing revealed that the mature 26 S, 17 S, 5.8 S, and 5 S rRNAs have sizes of 3741, 1871, 162, and 112 nucleotides, respectively. Unlike the published data, all mature rRNAs of the same type uniformly display the same start and end nucleotides in the analyzed AX2 strain. We show the existence of a short lived primary transcript covering the rRNA transcription unit of 17 S, 5.8 S, and 26 S rRNA. Northern blots and RT-PCR reveal that from this primary transcript two precursor molecules of the 17 S and two precursors of the 26 S rRNA are generated. We have also determined the sequences of these precursor molecules, and based on these data, we propose a model for the maturation of the rRNAs in Dictyostelium discoideum that we compare with the processing of the rRNA transcription unit of Saccharomyces cerevisiae.
Related Links	http://dx.doi.org/10.1074/jbc.m110.208306
Ending Page	17703
Page Count	11
Starting Page	17693
File Format	PDF
ISSN	00219258
e-ISSN	1083351X
Journal	The Journal of Biological Chemistry
Issue Number	20
Volume Number	286
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2011-05-20
Access Restriction	Open
Rights Holder	American Society for Biochemistry and Molecular Biology
Subject Keyword	Cell Biology Biochemistry Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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Sequence and Generation of Mature Ribosomal RNA Transcripts in Dictyostelium discoideum

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Sequence and Generation of Mature Ribosomal RNA Transcripts in Dictyostelium discoideum

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Sequence and Generation of Mature Ribosomal RNA Transcripts in Dictyostelium discoideum

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Sequence and Generation of Mature Ribosomal RNA Transcripts in Dictyostelium discoideum