NDLI: Intracellular sucrose communicates metabolic demand to sucrose transporters in developing pea cotyledons

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CORRIGENDUM

Year: 2008, Volume: 59

Year: 2007, Volume: 58

Intracellular sucrose communicates metabolic demand to sucrose transporters in developing pea cotyledons

Content Provider	PubMed Central
Author	Zhou, Yuchan Chan, Katie Wang, Trevor L. Hedley, Cliff L. Offler, Christina E. Patrick, John W.
Copyright Year	2009
Abstract	Mechanistic inter-relationships in sinks between sucrose compartmentation/metabolism and phloem unloading/translocation are poorly understood. Developing grain legume seeds provide tractable experimental systems to explore this question. Metabolic demand by cotyledons is communicated to phloem unloading and ultimately import by sucrose withdrawal from the seed apoplasmic space via a turgor-homeostat mechanism. What is unknown is how metabolic demand is communicated to cotyledon sucrose transporters responsible for withdrawing sucrose from the apoplasmic space. This question was explored here using a pea rugosus mutant (rrRbRb) compromised in starch biosynthesis compared with its wild-type counterpart (RRRbRb). Sucrose influx into cotyledons was found to account for 90% of developmental variations in their absolute growth and hence starch biosynthetic rates. Furthermore, rr and RR cotyledons shared identical response surfaces, indicating that control of transporter activity was likely to be similar for both lines. In this context, sucrose influx was correlated positively with expression of a sucrose/H+ symporter (PsSUT1) and negatively with two sucrose facilitators (PsSUF1 and PsSUF4). Sucrose influx exhibited a negative curvilinear relationship with cotyledon concentrations of sucrose and hexoses. In contrast, the impact of intracellular sugars on transporter expression was transporter dependent, with expression of PsSUT1 inhibited, PsSUF1 unaffected, and PsSUF4 enhanced by sugars. Sugar supply to, and sugar concentrations of, RR cotyledons were manipulated using in vitro pod and cotyledon culture. Collectively the results obtained showed that intracellular sucrose was the physiologically active sugar signal that communicated metabolic demand to sucrose influx and this transport function was primarily determined by PsSUT1 regulated at the transcriptional level.
Related Links	http://dx.doi.org/10.1093/jxb/ern254
Ending Page	85
Page Count	15
Starting Page	71
File Format	PDF
ISSN	00220957
e-ISSN	14602431
Journal	Journal of Experimental Botany
Issue Number	1
Volume Number	60
Language	English
Publisher	Oxford University Press
Publisher Date	2008-11-01
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Plant Science Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science

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