NDLI: Using zeta-potential measurements to quantify peptide partition to lipid membranes

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Using zeta-potential measurements to quantify peptide partition to lipid membranes

Content Provider	PubMed Central
Author	Freire, João M. Domingues, Marco M. Matos, Joana Melo, Manuel N. Veiga, Ana Salomé Santos, Nuno C. Castanho, Miguel A. R. B.
Abstract	Many cellular phenomena occur on the biomembranes. There are plenty of molecules (natural or xenobiotics) that interact directly or partially with the cell membrane. Biomolecules, such as several peptides (e.g., antimicrobial peptides) and proteins, exert their effects at the cell membrane level. This feature makes necessary investigating their interactions with lipids to clarify their mechanisms of action and side effects necessary. The determination of molecular lipid/water partition constants (K p ) is frequently used to quantify the extension of the interaction. The determination of this parameter has been achieved by using different methodologies, such as UV-Vis absorption spectrophotometry, fluorescence spectroscopy and ζ-potential measurements. In this work, we derived and tested a mathematical model to determine the K p from ζ-potential data. The values obtained with this method were compared with those obtained by fluorescence spectroscopy, which is a regular technique used to quantify the interaction of intrinsically fluorescent peptides with selected biomembrane model systems. Two antimicrobial peptides (BP100 and pepR) were evaluated by this new method. The results obtained by this new methodology show that ζ-potential is a powerful technique to quantify peptide/lipid interactions of a wide variety of charged molecules, overcoming some of the limitations inherent to other techniques, such as the need for fluorescent labeling.
Related Links	http://dx.doi.org/10.1007/s00249-010-0661-4
Ending Page	487
Page Count	7
Starting Page	481
File Format	PDF
ISSN	01757571
e-ISSN	14321017
Journal	European Biophysics Journal
Issue Number	4
Volume Number	40
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-04-01
Access Restriction	Open
Rights Holder	Springer-Verlag
Subject Keyword	Biophysics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine Biophysics

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