NDLI: Cloning, sequencing and expression of the Taq I restriction-modification system.

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Cloning, sequencing and expression of the Taq I restriction-modification system.

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Cloning, sequencing and expression of the Taq I restriction-modification system.

Content Provider	PubMed Central
Author	Slatko, B. E. Benner, J. S. Jager-quinton, T. Moran, L. S. Simcox, T. G. Cott, E. M. Van Wilson, G. G.
Abstract	The Taq I modification and restriction genes (recognition sequence TCGA) have been cloned in E. coli and their DNA sequences have been determined. Both proteins were characterized and the N-terminal sequence of the endonuclease was determined. The genes have the same transcriptional orientation with the methylase gene 5' to the endonuclease gene. The methylase gene is 1089 bp in length (363 amino acids, 40,576 daltons); the endonuclease gene is 702 bp in length (234 amino acids, 27,523 daltons); they are separated by 132 bp. Both methylase and endonuclease activity can be detected in cell extracts. The clones fully modify the vector and chromosomal DNA but they fail to restrict infecting phage. Clones carrying only the restriction gene are viable even in the absence of modification. The restriction gene contains 7 Taq I sites; the modification gene contains none. This asymmetric distribution of sites could be important in the regulation of the expression of the endonuclease gene.
Starting Page	9781
File Format	PDF
ISSN	13624962
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	23
Volume Number	15
Language	English
Publisher Date	1987-12-10
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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