NDLI: An Evolutionary Hot Spot: the pNGR234b Replicon of Rhizobium sp. Strain NGR234

Content Provider	PubMed Central
Author	Streit, W. R. Schmitz, R. A. Perret, X. Staehelin, C. Deakin, W. J. Raasch, C. Liesegang, H. Broughton, W. J.
Copyright Year	2004
Abstract	Rhizobium sp. strain NGR234 has an exceptionally broad host range and is able to nodulate more than 112 genera of legumes. Since the overall organization of the NGR234 genome is strikingly similar to that of the narrow-host-range symbiont Rhizobium meliloti strain 1021 (also known as Sinorhizobium meliloti), the obvious question is why are the spectra of hosts so different? Study of the early symbiotic genes of both bacteria (carried by the SymA plasmids) did not provide obvious answers. Yet, both rhizobia also possess second megaplasmids that bear, among many other genes, those that are involved in the synthesis of extracellular polysaccharides (EPSs). EPSs are involved in fine-tuning symbiotic interactions and thus may help answer the broad- versus narrow-host-range question. Accordingly, we sequenced two fragments (total, 594 kb) that encode 575 open reading frames (ORFs). Comparisons revealed 19 conserved gene clusters with high similarity to R. meliloti, suggesting that a minimum of 28% (158 ORFs) of the genetic information may have been acquired from a common ancestor. The largest conserved cluster carried the exo and exs genes and contained 31 ORFs. In addition, nine highly conserved regions with high similarity to Agrobacterium tumefaciens C58, Bradyrhizobium japonicum USDA110, and Mesorhizobium loti strain MAFF303099, as well as two conserved clusters that are highly homologous to similar regions in the plant pathogen Erwinia carotovora, were identified. Altogether, these findings suggest that ≥40% of the pNGR234b genes are not strain specific and were probably acquired from a wide variety of other microbes. The presence of 26 ORFs coding for transposases and site-specific integrases supports this contention. Surprisingly, several genes involved in the degradation of aromatic carbon sources and genes coding for a type IV pilus were also found.
Related Links	http://dx.doi.org/10.1128/jb.186.2.535-542.2004
Ending Page	542
Page Count	8
Starting Page	535
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	2
Volume Number	186
Language	English
Publisher	American Society for Microbiology
Publisher Date	2004-01-15
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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Functional and evolutionary relatedness of genes for exopolysaccharide synthesis in Rhizobium meliloti and Rhizobium sp. strain NGR234.

Dynamics of Genome Architecture in Rhizobium sp. Strain NGR234†

Rhizobium sp. strain NGR234 NodZ protein is a fucosyltransferase.

Three Replicons of Rhizobium sp. Strain NGR234 Harbor Symbiotic Gene Sequences

NolL of Rhizobium sp. Strain NGR234 Is Required for O-Acetyltransferase Activity

Characterization of NopP, a Type III Secreted Effector of Rhizobium sp. Strain NGR234

Role of BacA in Lipopolysaccharide Synthesis, Peptide Transport, and Nodulation by Rhizobium sp. Strain NGR234 ▿

Heterologous exopolysaccharide production in Rhizobium sp. strain NGR234 and consequences for nodule development.

Protein-Protein Interactions within Type III Secretion System-Dependent Pili of Rhizobium sp. Strain NGR234▿

An Evolutionary Hot Spot: the pNGR234b Replicon of Rhizobium sp. Strain NGR234

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Functional and evolutionary relatedness of genes for exopolysaccharide synthesis in Rhizobium meliloti and Rhizobium sp. strain NGR234.

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Rhizobium sp. strain NGR234 NodZ protein is a fucosyltransferase.

Three Replicons of Rhizobium sp. Strain NGR234 Harbor Symbiotic Gene Sequences

NolL of Rhizobium sp. Strain NGR234 Is Required for O-Acetyltransferase Activity

Characterization of NopP, a Type III Secreted Effector of Rhizobium sp. Strain NGR234

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Protein-Protein Interactions within Type III Secretion System-Dependent Pili of Rhizobium sp. Strain NGR234▿

An Evolutionary Hot Spot: the pNGR234b Replicon of Rhizobium sp. Strain NGR234