NDLI: Changes in Cationic Selectivity of the Nicotinic Channel at the Rat Ganglionic Synapse: A Role for Chloride Ions?

Content Provider	PubMed Central
Author	Sacchi, Oscar Rossi, Maria Lisa Canella, Rita Riccardo, Fesce
Editor	Brezina, Vladimir
Abstract	The permeability of the nicotinic channel (nAChR) at the ganglionic synapse has been examined, in the intact rat superior cervical ganglion in vitro, by fitting the Goldman current equation to the synaptic current (EPSC) I–V relationship. Subsynaptic nAChRs, activated by neurally-released acetylcholine (ACh), were thus analyzed in an intact environment as natively expressed by the mature sympathetic neuron. Postsynaptic neuron hyperpolarization (from −40 to −90 mV) resulted in a change of the synaptic potassium/sodium permeability ratio (PK/PNa) from 1.40 to 0.92, corresponding to a reversible shift of the apparent acetylcholine equilibrium potential, EACh, by about +10 mV. The effect was accompanied by a decrease of the peak synaptic conductance (gsyn) and of the EPSC decay time constant. Reduction of [Cl−]o to 18 mM resulted in a change of PK/PNa from 1.57 (control) to 2.26, associated with a reversible shift of EACh by about −10 mV. Application of 200 nM αBgTx evoked PK/PNa and gsyn modifications similar to those observed in reduced [Cl−]o. The two treatments were overlapping and complementary, as if the same site/mechanism were involved. The difference current before and after chloride reduction or toxin application exhibited a strongly positive equilibrium potential, which could not be explained by the block of a calcium component of the EPSC. Observations under current-clamp conditions suggest that the driving force modification of the EPSC due to PK/PNa changes represent an additional powerful integrative mechanism of neuron behavior. A possible role for chloride ions is suggested: the nAChR selectivity was actually reduced by increased chloride gradient (membrane hyperpolarization), while it was increased, moving towards a channel preferentially permeable for potassium, when the chloride gradient was reduced.
Related Links	http://dx.doi.org/10.1371/journal.pone.0017318
Starting Page	17318
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	2
Volume Number	6
Language	English
Publisher	Public Library of Science
Publisher Date	2011-02-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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