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| Content Provider | PubMed Central |
|---|---|
| Author | Watanabe, Tomonobu M. Fukui, Shingo Jin, Takashi Fujii, Fumihiko Yanagida, Toshio |
| Copyright Year | 2010 |
| Abstract | Superresolution optical microscopy (nanoscopy) is of current interest in many biological fields. Superresolution optical fluctuation imaging, which utilizes higher-order cumulant of fluorescence temporal fluctuations, is an excellent method for nanoscopy, as it requires neither complicated optics nor illuminations. However, it does need an impractical number of images for real-time observation. Here, we achieved real-time nanoscopy by modifying superresolution optical fluctuation imaging and enhancing the fluctuation of quantum dots. Our developed quantum dots have higher blinking than commercially available ones. The fluctuation of the blinking improved the resolution when using a variance calculation for each pixel instead of a cumulant calculation. This enabled us to obtain microscopic images with 90-nm and 80-ms spatial-temporal resolution by using a conventional fluorescence microscope without any optics or devices. |
| Related Links | http://dx.doi.org/10.1016/j.bpj.2010.07.036 |
| Starting Page | 50 |
| File Format | |
| ISSN | 00063495 |
| e-ISSN | 15420086 |
| Journal | Biophysical Journal |
| Issue Number | 7 |
| Volume Number | 99 |
| Language | English |
| Publisher | The Biophysical Society |
| Publisher Date | 2010-10-01 |
| Access Restriction | Open |
| Rights Holder | The Biophysical Society |
| Subject Keyword | Biophysics Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biophysics |
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