NDLI: Effect of Population Heterogenization on the Reproducibility of Mouse Behavior: A Multi-Laboratory Study

Content Provider	PubMed Central
Author	Richter, S. Helene Garner, Joseph P. Zipser, Benjamin Lewejohann, Lars Sachser, Norbert Touma, Chadi Schindler, Britta Sabine, Chourbaji Brandwein, Christiane Gass, Peter Stipdonk, Niek Van Harst, Johanneke Van Der Berry, Spruijt Võikar, Vootele Wolfer, David P. Hanno, Würbel
Editor	Chapouthier, Georges
Abstract	In animal experiments, animals, husbandry and test procedures are traditionally standardized to maximize test sensitivity and minimize animal use, assuming that this will also guarantee reproducibility. However, by reducing within-experiment variation, standardization may limit inference to the specific experimental conditions. Indeed, we have recently shown in mice that standardization may generate spurious results in behavioral tests, accounting for poor reproducibility, and that this can be avoided by population heterogenization through systematic variation of experimental conditions. Here, we examined whether a simple form of heterogenization effectively improves reproducibility of test results in a multi-laboratory situation. Each of six laboratories independently ordered 64 female mice of two inbred strains (C57BL/6NCrl, DBA/2NCrl) and examined them for strain differences in five commonly used behavioral tests under two different experimental designs. In the standardized design, experimental conditions were standardized as much as possible in each laboratory, while they were systematically varied with respect to the animals' test age and cage enrichment in the heterogenized design. Although heterogenization tended to improve reproducibility by increasing within-experiment variation relative to between-experiment variation, the effect was too weak to account for the large variation between laboratories. However, our findings confirm the potential of systematic heterogenization for improving reproducibility of animal experiments and highlight the need for effective and practicable heterogenization strategies.
Related Links	http://dx.doi.org/10.1371/journal.pone.0016461
Starting Page	16461
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	1
Volume Number	6
Language	English
Publisher	Public Library of Science
Publisher Date	2011-01-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Risk Factors for Methicillin Resistant Staphylococcus aureus: A Multi-Laboratory Study

Toward a Mouse Neuroethology in the Laboratory Environment

Reproducibility in Echocardiographic Assessment of Diastolic Function in a Population Based Study (The STANISLAS Cohort Study)

Effect of Soil Moisture Content on the Splash Phenomenon Reproducibility

Reproducibility of Frankfort Horizontal Plane on 3D Multi-Planar Reconstructed MR Images

Correction: Estimating the Inbreeding Depression on Cognitive Behavior: A Population Based Study of Child Cohort

The Effect of Cage Space on Behavior and Reproduction in Crl:CD1(Icr) and C57BL/6NCrl Laboratory Mice

The Leverage Effect on Wealth Distribution in a Controllable Laboratory Stock Market

Effect of Cigarette Smoking and Passive Smoking on Hearing Impairment: Data from a Population–Based Study

Effect of Population Heterogenization on the Reproducibility of Mouse Behavior: A Multi-Laboratory Study

Similar Documents

Risk Factors for Methicillin Resistant Staphylococcus aureus: A Multi-Laboratory Study

Toward a Mouse Neuroethology in the Laboratory Environment

Reproducibility in Echocardiographic Assessment of Diastolic Function in a Population Based Study (The STANISLAS Cohort Study)

Effect of Soil Moisture Content on the Splash Phenomenon Reproducibility

Reproducibility of Frankfort Horizontal Plane on 3D Multi-Planar Reconstructed MR Images

Correction: Estimating the Inbreeding Depression on Cognitive Behavior: A Population Based Study of Child Cohort

The Effect of Cage Space on Behavior and Reproduction in Crl:CD1(Icr) and C57BL/6NCrl Laboratory Mice

The Leverage Effect on Wealth Distribution in a Controllable Laboratory Stock Market

Effect of Cigarette Smoking and Passive Smoking on Hearing Impairment: Data from a Population–Based Study

Effect of Population Heterogenization on the Reproducibility of Mouse Behavior: A Multi-Laboratory Study