NDLI: Transgene Expression and Transgene-Induced Silencing in Diploid and Autotetraploid Arabidopsis

Content Provider	PubMed Central
Author	Finn, Thomas E. Wang, Lei David, Smolilo Smith, Neil A. White, Rosemary Chaudhury, Abed Dennis, Elizabeth S. Wang, Ming-bo
Copyright Year	2011
Abstract	Previous studies have suggested that transgene expression in plants can be affected by ploidy. Here we show that three different transgenes, a reporter transgene, an antisense transgene, and a hairpin RNA (hpRNA) transgene, are all expressed at a lower level in autotetraploid (4n) than in diploid (2n) Arabidopsis. RNA silencing of two endogenous genes was induced by the antisense and hpRNA transgenes and this silencing is significantly less effective in 4n than in 2n Arabidopsis; furthermore, the reduced silencing in 4n Arabidopsis correlated with reduced accumulation of silencing-inducer RNAs. Methylation analysis both of independent 2n and 4n transgenic lines and of 2n and 4n progeny derived from the same 3n transgenic parent, indicated that transgenes are more methylated in 4n than 2n Arabidopsis. These results suggest that transgenes are transcriptionally repressed in the 4n background, resulting in expression levels lower than in the 2n background. Transgenes designed to silence endogenous genes express lower concentrations of silencing-inducer RNAs in 4n Arabidopsis plants, resulting in less effective silencing of target genes than in 2n Arabidopsis plants.
Related Links	http://dx.doi.org/10.1534/genetics.110.124370
Ending Page	423
Page Count	15
Starting Page	409
File Format	PDF
ISSN	00166731
e-ISSN	19432631
Journal	Genetics
Issue Number	2
Volume Number	187
Language	English
Publisher	Genetics Society of America
Publisher Date	2011-02-01
Access Restriction	Open
Rights Holder	Genetics Society of America
Subject Keyword	Genetics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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