NDLI: Role for topoisomerase 1 in transcription-associated mutagenesis in yeast

Content Provider	PubMed Central
Author	Lippert, Malcolm J. Kim, Nayun Cho, Jang-eun Larson, Ryan P. Schoenly, Nathan E. O'shea, Shannon H. Sue, Jinks-robertson
Abstract	High levels of transcription in Saccharomyces cerevisiae are associated with increased genetic instability, which has been linked to DNA damage. Here, we describe a pGAL-CAN1 forward mutation assay for studying transcription-associated mutagenesis (TAM) in yeast. In a wild-type background with no alterations in DNA repair capacity, ≈50% of forward mutations that arise in the CAN1 gene under high-transcription conditions are deletions of 2–5 bp. Furthermore, the deletions characteristic of TAM localize to discrete hotspots that coincide with 2–4 copies of a tandem repeat. Although the signature deletions of TAM are not affected by the loss of error-free or error-prone lesion bypass pathways, they are completely eliminated by deletion of the TOP1 gene, which encodes the yeast type IB topoisomerase. Hotspots can be transposed into the context of a frameshift reversion assay, which is sensitive enough to detect Top1-dependent deletions even in the absence of high transcription. We suggest that the accumulation of Top1 cleavage complexes is related to the level of transcription and that their removal leads to the signature deletions. Given the high degree of conservation between DNA metabolic processes, the links established here among transcription, Top1, and mutagenesis are likely to extend beyond the yeast system.
Related Links	http://dx.doi.org/10.1073/pnas.1012363108
Ending Page	703
Page Count	6
Starting Page	698
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	2
Volume Number	108
Language	English
Publisher	National Academy of Sciences
Publisher Date	2011-01-11
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Role for topoisomerase 1 in transcription-associated mutagenesis in yeast.

A role for noncoding transcription in activation of the yeast PHO5 gene

Poxvirus DNA topoisomerase knockout mutant exhibits decreased infectivity associated with reduced early transcription

Metabolic role for yeast DJ-1 superfamily proteins

Two distinct mechanisms of Topoisomerase 1-dependent mutagenesis in yeast

Topoisomerase 1 provokes the formation of short deletions in repeated sequences upon high transcription in Saccharomyces cerevisiae

Role of DNA topoisomerase I in the transcription of supercoiled rRNA gene.

Statistical methods for identifying yeast cell cycle transcription factors

Topoisomerase II binds nucleosome-free DNA and acts redundantly with topoisomerase I to enhance recruitment of RNA Pol II in budding yeast

Role for topoisomerase 1 in transcription-associated mutagenesis in yeast

Similar Documents

Role for topoisomerase 1 in transcription-associated mutagenesis in yeast.

A role for noncoding transcription in activation of the yeast PHO5 gene

Poxvirus DNA topoisomerase knockout mutant exhibits decreased infectivity associated with reduced early transcription

Metabolic role for yeast DJ-1 superfamily proteins

Two distinct mechanisms of Topoisomerase 1-dependent mutagenesis in yeast

Topoisomerase 1 provokes the formation of short deletions in repeated sequences upon high transcription in Saccharomyces cerevisiae

Role of DNA topoisomerase I in the transcription of supercoiled rRNA gene.

Statistical methods for identifying yeast cell cycle transcription factors

Topoisomerase II binds nucleosome-free DNA and acts redundantly with topoisomerase I to enhance recruitment of RNA Pol II in budding yeast

Role for topoisomerase 1 in transcription-associated mutagenesis in yeast