NDLI: Characterization of the Bacteroides CTnDOT Regulatory Protein RteC▿

Content Provider	PubMed Central
Author	Park, Jiyeon Salyers, Abigail A.
Copyright Year	2011
Abstract	Excision of the Bacteroides conjugative transposon CTnDOT is stimulated by tetracycline. It was shown previously that a gene, rteC, is necessary for tetracycline-stimulated transcriptional regulation of the orf2c operon, which contains the excision genes. The protein encoded by this gene, RteC, did not have primary amino acid sequence homology to any known proteins in the databases. Accordingly, we sought structural homologs of RteC. A three-dimensional structure prediction by Robetta suggested that RteC might have two domains and that the C-terminal domain might have a winged helix motif. Based on the Robetta prediction, the human transcriptional factors E2F-4 and DP2 were identified as the most likely structural homologs of RteC. We made alanine substitutions within the putative DNA binding helix 3 region of RteC. Assays of orf2c::uidA activation by alanine mutants indicated that residues 174, 175, 178, 180, and 184 in helix 3 might contact the upstream region of PE. The upstream region of orf2c contained two inverted-repeat half sites. Mutational analysis of these half sites showed that both half sites are important for activity. Thus, we have identified the DNA binding portion of RteC and the DNA site to which it binds.
Related Links	http://dx.doi.org/10.1128/jb.01015-10
Ending Page	97
Page Count	7
Starting Page	91
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	1
Volume Number	193
Language	English
Publisher	American Society for Microbiology (ASM)
Publisher Date	2011-01-01
Access Restriction	Open
Rights Holder	American Society for Microbiology (ASM)
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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Characterization of Genes Involved in Modulation of Conjugal Transfer of the Bacteroides Conjugative Transposon CTnDOT

The Bacteroides thetaiotaomicron Protein Bacteroides Host Factor A Participates in Integration of the Integrative Conjugative Element CTnDOT into the Chromosome

Regulation of Excision Genes of the Bacteroides Conjugative Transposon CTnDOT

Development of an In Vitro Integration Assay for the Bacteroides Conjugative Transposon CTnDOT

Translational Control of Tetracycline Resistance and Conjugation in the Bacteroides Conjugative Transposon CTnDOT

Tetracycline-Associated Transcriptional Regulation of Transfer Genes of the Bacteroides Conjugative Transposon CTnDOT▿

Integration and Excision of a Bacteroides Conjugative Transposon, CTnDOT

The Small RNA RteR Inhibits Transfer of the Bacteroides Conjugative Transposon CTnDOT

Regulation of a Bacteroides Operon That Controls Excision and Transfer of the Conjugative Transposon CTnDOT

Characterization of the Bacteroides CTnDOT Regulatory Protein RteC▿

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