NDLI: Splicing-Dependent RNA Polymerase Pausing in Yeast

Content Provider	PubMed Central
Author	Alexander, Ross D. Innocente, Steven A. Barrass, J. David Beggs, Jean D.
Copyright Year	2010
Abstract	In eukaryotic cells, there is evidence for functional coupling between transcription and processing of pre-mRNAs. To better understand this coupling, we performed a high-resolution kinetic analysis of transcription and splicing in budding yeast. This revealed that shortly after induction of transcription, RNA polymerase accumulates transiently around the 3′ end of the intron on two reporter genes. This apparent transcriptional pause coincides with splicing factor recruitment and with the first detection of spliced mRNA and is repeated periodically thereafter. Pausing requires productive splicing, as it is lost upon mutation of the intron and restored by suppressing the splicing defect. The carboxy-terminal domain of the paused polymerase large subunit is hyperphosphorylated on serine 5, and phosphorylation of serine 2 is first detected here. Phosphorylated polymerase also accumulates around the 3′ splice sites of constitutively expressed, endogenous yeast genes. We propose that transcriptional pausing is imposed by a checkpoint associated with cotranscriptional splicing.
Related Links	http://dx.doi.org/10.1016/j.molcel.2010.11.005
Ending Page	593
Page Count	12
Starting Page	582
File Format	PDF
ISSN	10972765
e-ISSN	10974164
Journal	Molecular Cell
Issue Number	4
Volume Number	40
Language	English
Publisher	Cell Press
Publisher Date	2010-11-01
Access Restriction	Open
Rights Holder	Cell Press
Subject Keyword	Cell Biology Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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