NDLI: Studies on the structure and dynamics of the human telomeric G quadruplex by single-molecule fluorescence resonance energy transfer

Content Provider	PubMed Central
Author	Ying, Liming Green, Jeremy J. Li, Haitao Klenerman, David Balasubramanian, Shankar
Copyright Year	2003
Abstract	We have investigated the structure and unfolding kinetics of the human telomeric intramolecular G quadruplex by using single-molecule fluorescence resonance energy transfer. An exploration of conformational heterogeneity revealed two stable folded conformations, in both sodium- and potassium-containing buffers, with small differences between their enthalpies and entropies. Both folded conformations can be opened by the addition of a 21-base complementary DNA oligonucleotide. The unfolding of both substates occurs at the same rate, which showed dependence on the monovalent metal cation present. Temperature-dependence studies in 100 mM KCl gave an apparent activation enthalpy and entropy of 6.4 ± 0.4 kcal·mol–1 and –52.3 ± 1.4 cal·mol–1·K–1, respectively, indicating that the unfolding is entropically driven and can occur easily. In contrast, in 100 mM NaCl the respective values are 14.9 ± 0.2 kcal·mol–1 and –23.0 ± 0.8 cal·mol–1·K–1, suggesting a more significant enthalpic barrier. Molecular modeling suggests that the two species are likely to be the parallel and antiparallel quadruplex structures. The unfolding free energy barrier is estimated to be between 3 and 15 k B T based on Kramers' theory. We conclude that under near-physiological conditions these structures coexist and can interconvert on a minute time scale.
Related Links	http://dx.doi.org/10.1073/pnas.2433350100
Ending Page	14634
Page Count	6
Starting Page	14629
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	25
Volume Number	100
Language	English
Publisher	National Academy of Sciences
Publisher Date	2003-12-09
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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