NDLI: Identification of a distal tandem STAT6 element within the CCL17 locus

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Identification of a distal tandem STAT6 element within the CCL17 locus

Content Provider	PubMed Central
Author	Maier, Elisabeth Wirnsberger, Gerald Horejs-hoeck, Jutta Albert, Duschl Hebenstreit, Daniel
Copyright Year	2007
Abstract	The CC chemokine ligand CCL17 is one of the major chemo-attractants for TH2 cells. Interleukin-4–induced activation of CCL17 expression was recently demonstrated to result from two STAT6 motifs in the proximal promoter. Here we provide evidence that a distal tandem STAT6 element further elevates expression from the CCL17 locus approximately twofold. This is demonstrated by reporter gene assays using different fragments of the CCL17 promoter and the region 2.5 kb upstream from the transcriptional start site. By electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation experiments, we show that STAT6 binds to the motifs in vitro and in vivo, respectively. Insertion of nucleotide exchanges into the STAT6 core motifs results in diminished promoter activation and abrogated STAT6 binding, as demonstrated by reporter gene and EMSA studies. Collectively these data reveal an additional element involved in the regulation of CCL17 expression.
Related Links	http://dx.doi.org/10.1016/j.humimm.2007.10.012
Starting Page	986
File Format	PDF
ISSN	18791166
e-ISSN	18791166
Journal	Human immunology
Issue Number	12
Volume Number	68
Language	English
Publisher Date	2007-12-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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