NDLI: Purification of the Formate-Tetrahydrofolate Ligase from Methylobacterium extorquens AM1 and Demonstration of Its Requirement for Methylotrophic Growth

Content Provider	PubMed Central
Author	Marx, Christopher J. Markus, Laukel Vorholt, Julia A. Lidstrom, Mary E.
Copyright Year	2003
Abstract	The serine cycle methylotroph Methylobacterium extorquens AM1 contains two pterin-dependent pathways for C1 transfers, the tetrahydrofolate (H4F) pathway and the tetrahydromethanopterin (H4MPT) pathway, and both are required for growth on C1 compounds. With the exception of formate-tetrahydrofolate ligase (FtfL, alternatively termed formyl-H4F synthetase), all of the genes encoding the enzymes comprising these two pathways have been identified, and the corresponding gene products have been purified and characterized. We present here the purification and characterization of FtfL from M. extorquens AM1 and the confirmation that this enzyme is encoded by an ftfL homolog identified previously through transposon mutagenesis. Phenotypic analyses of the ftfL mutant strain demonstrated that FtfL activity is required for growth on C1 compounds. Unlike mutants defective for the H4MPT pathway, the ftfL mutant strain does not exhibit phenotypes indicative of defective formaldehyde oxidation. Furthermore, the ftfL mutant strain remained competent for wild-type conversion of [14C]methanol to [14C]CO2. Collectively, these data confirm our previous presumptions that the H4F pathway is not the key formaldehyde oxidation pathway in M. extorquens AM1. Rather, our data suggest an alternative model for the role of the H4F pathway in this organism in which it functions to convert formate to methylene H4F for assimilatory metabolism.
Related Links	http://dx.doi.org/10.1128/JB.185.24.7169-7175.2003
Starting Page	7169
File Format	PDF
ISSN	10985530
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	24
Volume Number	185
Language	English
Publisher	American Society for Microbiology
Publisher Date	2003-12-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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