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| Content Provider | PubMed Central |
|---|---|
| Author | Borgmann-winter, K. E. Rawson, N. E. Wang, Hoau-yan Wang, H. Macdonald, M. L. Ozdener, M. H. Yee, K. K. Gomez, G. Xu, J. Bryant, B. Adamek, G. Mirza, N. Pribitkin, E. Hahn, C-g. |
| Abstract | The olfactory epithelium constitutes the sole source of regenerating neural cells that can be obtained from a living human. As such, primary cultures derived from olfactory epithelial biopsies can be utilized to study neurobiological characteristics of individuals under different conditions and disease states. Here, using such cultures, we report in vitro generation of cells that exhibit a complex neuronal phenotype, encompassing receptors and signaling pathways pertinent to both olfaction and other aspects of CNS function. Using in situ hybridization, we demonstrate for the first time the native expression of olfactory receptors in cultured cells derived from olfactory epithelial tissue. We further establish the presence and function of olfactory transduction molecules in these cells using immunocytochemistry, calcium imaging and molecular methods. Western blot analysis revealed the expression of neurotransmitter receptors for dopamine (D2R), serotonin (5HT2C) and NMDA subtypes 1 and 2A/2B. Stimulation with dopamine or serotonin enhanced receptor G protein activation in a subtype specific manner, based on 35S-GTP incorporation assay. Functional characteristics of the cultured cells are demonstrated through enhanced tyrosine phosphorylation of NMDAR 2A/2B and recruitment of signaling partners in response to NMDA stimulation. The array of neuronal characteristics observed here establish that proliferating cells derived from the human olfactory epithelium differentiate in vitro to express functional and molecular attributes of mature olfactory neurons. These cultured neural cells exhibit neurotransmitter pathways important in a number of neuropsychiatric disorders. Their ready availability from living humans thus provides a new tool to link functional and molecular features of neural cells with clinical characteristics of individual living patients. |
| Related Links | http://dx.doi.org/10.1016/j.neuroscience.2008.09.059 |
| Ending Page | 653 |
| Page Count | 12 |
| Starting Page | 642 |
| File Format | |
| ISSN | 03064522 |
| Journal | Neuroscience |
| Issue Number | 2 |
| Volume Number | 158 |
| Language | English |
| Publisher Date | 2009-01-01 |
| Access Restriction | Open |
| Subject Keyword | Neuroscience(all) Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Neuroscience |
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