NDLI: ICAM-1 enrichment near tri-cellular endothelial junctions is preferentially associated with leukocyte transmigration, and signals for reorganization of these junctions to accommodate leukocyte passage

Content Provider	PubMed Central
Author	Ronen, Sumagin Sarelius, Ingrid H.
Abstract	Leukocyte transmigration occurs at specific locations (portals) on the endothelium, but the nature of these portals is not clear. Using intravital confocal microscopy of anesthetized mouse cremaster muscle in combination with immunofluorescence labeling, we showed that in microvessels transmigration is mainly junctional and preferentially occurs at tri-cellular endothelial junctional regions. Our data suggest that enrichment of ICAM-1 near approximately 43% of these junctions makes these locations preferred for transmigration, by signaling the location of a nearby portal, as well as preparing the EC-junctions to accommodate leukocyte passage. Blockade of the extracellular domain of the ICAM-1 significantly reduced transmigration (by 68.8±4.5%), by reducing the ability of leukocytes to get to these portals. In contrast, blockade of the cytoplasmic tail of ICAM-1 reduced transmigration (by 71.1±7.0%) by disabling VE-Cadherin rearrangement. Importantly, venular convergences are optimally equipped to support leukocyte transmigration. Differences in EC morphology result in a significantly higher number of tri-cellular junctions in convergences compared to straight venular regions (20.7±1.2 vs 12.43±1.1/6000μm2, respectively). Consequently leukocyte adhesion and transmigration are significantly higher in convergences compared to straight regions (1.6- and 2.6-fold, respectively). Together, these data identify an important role for EC morphology and expression patterns of ICAM-1 in leukocyte transmigration.
Related Links	http://dx.doi.org/10.4049/jimmunol.0903319
Ending Page	5252
Page Count	11
Starting Page	5242
File Format	PDF
ISSN	00221767
e-ISSN	15506606
Journal	Journal of immunology (Baltimore, Md. : 1950)
Issue Number	9
Volume Number	184
Language	English
Publisher Date	2010-05-01
Access Restriction	Open
Subject Keyword	Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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