NDLI: Effects of cardiac overexpression of type 6 adenylyl cyclase affects on the response to chronic pressure overload

Content Provider	PubMed Central
Author	Aziz, Guellich Gao, Shumin Hong, Chull Yan, Lin Wagner, Thomas E. Dhar, Sunil K. Bijan, Ghaleh Hittinger, Luc Iwatsubo, Kosaku Ishikawa, Yoshihiro Vatner, Stephen F. Vatner, Dorothy E.
Abstract	Adenylyl cyclase (AC) type 5 (AC5) and AC type 6 (AC6) are the two major AC isoforms in the heart. Cardiac overexpression of AC6 has been shown to be protective in response to several interventions. In this investigation, we examined the effects of chronic pressure overload in AC6 transgenic (TG) mice. In the absence of any stress, AC6 TG mice exhibited enhanced contractile function compared with their wild-type (WT) littermates, i.e., increased (P < 0.05) left ventricular (LV) ejection fraction (EF) (75 ± 0.9 vs. 71 ± 0.5%) and LV dP/dt (7,850 ± 526 vs. 6,374 ± 315 mmHg/s). Forskolin (25 μg·kg−1·min−1 for 5 min) increased LVEF more (P < 0.05) in AC6 TG mice (14.8 ± 1.0%) than in WT mice (7.7 ± 1.0%). Also, isoproterenol (0.04 μg·kg−1·min−1 for 5 min) increased LVEF more (P < 0.05) in AC6 TG mice (18.0 ± 1.2%) than in WT mice (11.6 ± 2.1%). Pressure overload, induced by 4 wk of transverse aortic constriction (TAC), increased the LV weight-to-body weight ratio and myocyte cross-sectional area similarly in both groups, but reduced LVEF more in AC6 TG mice (22%) compared with WT mice (9%), despite the higher starting level of LVEF in AC6 TG mice. LV systolic wall stress increased more in AC6 TG mice than in WT mice, which could be responsible for the reduced LVEF in AC6 TG mice with chronic pressure overload. In addition, LV dP/dt was no longer elevated in AC6 TG mice after TAC compared with WT mice. LV end-diastolic diameter was also greater (P < 0.05) in AC6 TG mice (3.8 ± 0.07 mm) than in WT mice (3.6 ± 0.05 mm) after TAC. Thus, in contrast to other interventions previously reported to be salutary with cardiac AC6 overpression, the response to chronic pressure overload was not; actually, AC6 TG mice fared worse than WT mice. The mechanism may be due to the increased LV systolic wall stress in AC6 TG mice with chronic pressure overload.
Related Links	http://dx.doi.org/10.1152/ajpheart.00148.2010
Starting Page	707
File Format	PDF
ISSN	03636135
e-ISSN	15221539
Journal	American Journal of Physiology - Heart and Circulatory Physiology
Issue Number	3
Volume Number	299
Language	English
Publisher	American Physiological Society
Publisher Date	2010-09-01
Access Restriction	Open
Rights Holder	American Physiological Society
Subject Keyword	Physiology (medical) Physiology Cardiology and Cardiovascular Medicine Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Cardiology and Cardiovascular Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Effects of cardiac overexpression of type 6 adenylyl cyclase affects on the response to chronic pressure overload.

Disruption of adenylyl cyclase type V does not rescue the phenotype of cardiac-specific overexpression of Gαq protein-induced cardiomyopathy

Parent-of-origin effects on cardiac response to pressure overload in mice

Overexpression of adenylyl cyclase type 5 (AC5) confers a proarrhythmic substrate to the heart

Substrain specific response to cardiac pressure overload in C57BL/6 mice

Adenylyl cyclase type 5 protein expression during cardiac development and stress

Remodeling of the peripheral cardiac conduction system in response to pressure overload

Adenylyl cyclase type 5 in cardiac disease, metabolism, and aging

Cardiac-restricted overexpression or deletion of tissue inhibitor of matrix metalloproteinase-4: differential effects on left ventricular structure and function following pressure overload-induced hypertrophy

Effects of cardiac overexpression of type 6 adenylyl cyclase affects on the response to chronic pressure overload

Similar Documents

Effects of cardiac overexpression of type 6 adenylyl cyclase affects on the response to chronic pressure overload.

Disruption of adenylyl cyclase type V does not rescue the phenotype of cardiac-specific overexpression of Gαq protein-induced cardiomyopathy

Parent-of-origin effects on cardiac response to pressure overload in mice

Overexpression of adenylyl cyclase type 5 (AC5) confers a proarrhythmic substrate to the heart

Substrain specific response to cardiac pressure overload in C57BL/6 mice

Adenylyl cyclase type 5 protein expression during cardiac development and stress

Remodeling of the peripheral cardiac conduction system in response to pressure overload

Adenylyl cyclase type 5 in cardiac disease, metabolism, and aging

Cardiac-restricted overexpression or deletion of tissue inhibitor of matrix metalloproteinase-4: differential effects on left ventricular structure and function following pressure overload-induced hypertrophy

Effects of cardiac overexpression of type 6 adenylyl cyclase affects on the response to chronic pressure overload