NDLI: Transcellular Passage of Neisseria meningitidis across a Polarized Respiratory Epithelium▿ †

Content Provider	PubMed Central
Author	Sutherland, Thomas C. Quattroni, Paola Exley, Rachel M. Tang, Christoph M.
Copyright Year	2010
Abstract	Neisseria meningitidis is a major cause of sepsis and meningitis but is also a common commensal, present in the nasopharynx of between 8 and 20% of healthy individuals. During carriage, the bacterium is found on the surface of the nasopharyngeal epithelium and in deeper tissues, while to develop disease the meningococcus must spread across the respiratory epithelium and enter the systemic circulation. Therefore, investigating the pathways by which N. meningitidis crosses the epithelial barrier is relevant for understanding carriage and disease but has been hindered by the lack of appropriate models. Here, we have established a physiologically relevant model of the upper respiratory epithelial cell barrier to investigate the mechanisms responsible for traversal of N. meningitidis. Calu-3 human respiratory epithelial cells were grown on permeable cell culture membranes to form polarized monolayers of cells joined by tight junctions. We show that the meningococcus crosses the epithelial cell barrier by a transcellular route; traversal of the layer did not disrupt its integrity, and bacteria were detected within the cells of the monolayer. We demonstrate that successful traversal of the epithelial cell barrier by N. meningitidis requires expression of its type 4 pili (Tfp) and capsule and is dependent on the host cell microtubule network. The Calu-3 model should be suitable for dissecting the pathogenesis of infections caused by other respiratory pathogens, as well as the meningococcus.
Related Links	http://dx.doi.org/10.1128/iai.01377-09
Ending Page	3847
Page Count	16
Starting Page	3832
File Format	PDF
ISSN	00199567
e-ISSN	10985522
Journal	Infection and Immunity
Issue Number	9
Volume Number	78
Language	English
Publisher	American Society for Microbiology (ASM)
Publisher Date	2010-09-01
Access Restriction	Open
Rights Holder	American Society for Microbiology (ASM)
Subject Keyword	Immunology Microbiology Parasitology Infectious Diseases Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology Immunology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Transcellular Passage of Neisseria gonorrhoeae Involves Pilus Phase Variation

Interaction of Neisseria meningitidis with a polarized monolayer of epithelial cells.

A tissue culture bilayer model to study the passage of Neisseria meningitidis.

Analysis of damage to human ciliated nasopharyngeal epithelium by Neisseria meningitidis.

Mutations Affecting Peptidoglycan Acetylation in Neisseria gonorrhoeae and Neisseria meningitidis

Biofilm Formation by Neisseria meningitidis

Peptidoglycan Fragment Release from Neisseria meningitidis

Differential Role of Lipooligosaccharide of Neisseria meningitidis in Virulence and Inflammatory Response during Respiratory Infection in Mice

NadA Diversity and Carriage in Neisseria meningitidis

Transcellular Passage of Neisseria meningitidis across a Polarized Respiratory Epithelium▿ †

Similar Documents

Transcellular Passage of Neisseria gonorrhoeae Involves Pilus Phase Variation

Interaction of Neisseria meningitidis with a polarized monolayer of epithelial cells.

A tissue culture bilayer model to study the passage of Neisseria meningitidis.

Analysis of damage to human ciliated nasopharyngeal epithelium by Neisseria meningitidis.

Mutations Affecting Peptidoglycan Acetylation in Neisseria gonorrhoeae and Neisseria meningitidis

Biofilm Formation by Neisseria meningitidis

Peptidoglycan Fragment Release from Neisseria meningitidis

Differential Role of Lipooligosaccharide of Neisseria meningitidis in Virulence and Inflammatory Response during Respiratory Infection in Mice

NadA Diversity and Carriage in Neisseria meningitidis

Transcellular Passage of Neisseria meningitidis across a Polarized Respiratory Epithelium▿ †