NDLI: TNF-α-Induced Mitochondrial Alterations in Human T Cells Requires FADD and Caspase-8 Activation but Not RIP and Caspase-3 Activation

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TNF-α-Induced Mitochondrial Alterations in Human T Cells Requires FADD and Caspase-8 Activation but Not RIP and Caspase-3 Activation

Content Provider	PubMed Central
Author	Mehdi, Shakibaei Sung, Bokyung Sethi, Gautam Aggarwal, Bharat B.
Abstract	Although much is known about how TNF-α induces apoptosis in the presence of inhibitors of protein synthesis, little is known about how it induces apoptosis without these inhibitors. In this report we investigated temporal sequence of events induced by TNF-α in the absence of protein synthesis. Regardless of whether we measured the effects by plasma membrane phosphotidylserine accumulation, by DNA strand breaks, or activation of caspases, significant changes were observed only between 12–24 h of TNF-α treatment. One of the earliest changes observed after TNF-α treatment was mitochondrial swelling at 10 min; followed by cytochrome c and Smac release at 10–30 min, and then heterochromatin clumping occurred at 60 min. While genetic deletion of receptor-interaction protein (RIP) had no effect on TNF-α-induced mitochondrial damage, deletion of Fas-associated death domain (FADD) abolished the TNF-induced mitochondrial swelling. Since pan-caspase inhibitor z-VAD-fmk abolished the TNF-α-induced mitochondrial changes, z-DEVD-fmk, an inhibitor of caspase-3 had no effect, suggesting that TNF-α-induced mitochondrial changes or cytochrome c and Smac release requires caspase-8 but not caspase-3 activation. Overall, our results indicated that mitochondrial changes are early events in TNF-α-induced apoptosis and that these mitochondrial changes require recruitment of FADD and caspase-8 activation, but not caspase-3 activation or RIP recruitment. Antioxid. Redox Signal. 13, 821–831.
Related Links	http://dx.doi.org/10.1089/ars.2009.3036
Ending Page	831
Page Count	11
Starting Page	821
File Format	PDF
ISSN	15577716
e-ISSN	15577716
Journal	Antioxidants & Redox Signaling
Issue Number	6
Volume Number	13
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2010-09-15
Access Restriction	Open
Rights Holder	Mary Ann Liebert, Inc.
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Physiology Medicine Molecular Biology Clinical Biochemistry Biochemistry

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