NDLI: Identification and Mechanism of ABA Receptor Antagonism

Content Provider	PubMed Central
Author	Melcher, Karsten Xu, Yong Ng, Ley-moy Zhou, X. Edward Soon, Fen-fen Viswanathan, Chinnusamy Suino-powell, Kelly M. Kovach, Amanda Tham, Fook S. Cutler, Sean R. Li, Jun Yong, Eu-leong Zhu, Jian-kang Xu, H. Eric
Abstract	The phytohormone abscisic acid (ABA) functions through a family of fourteen PYR/PYL receptors, which were identified by resistance to pyrabactin, a synthetic inhibitor of seed germination. ABA activates these receptors to inhibit type 2C protein phosphatases, such as ABI1, yet it remains unclear whether these receptors can be antagonized. Here we demonstrate that pyrabactin is an agonist of PYR1 and PYL1, but unexpectedly an antagonist of PYL2. Crystal structures of the PYL2–pyrabactin and PYL1–pyrabactin–ABI1 complexes reveal the mechanism responsible for receptor-selective activation and inhibition, which enables us to design mutations that convert PYL1 to a pyrabactin-inhibited receptor and PYL2 to a pyrabactin-activated receptor, and to identify new pyrabactin-based ABA receptor agonists. Together, our results establish a new concept of ABA receptor antagonism, illustrate its underlying mechanisms, and provide a rational framework for discovering novel ABA receptor ligands.
Related Links	http://dx.doi.org/10.1038/nsmb.1887
Ending Page	1108
Page Count	7
Starting Page	1102
File Format	PDF
ISSN	15459993
e-ISSN	15459985
Journal	Nature structural & molecular biology
Issue Number	9
Volume Number	17
Language	English
Publisher Date	2010-09-01
Access Restriction	Open
Subject Keyword	Molecular Biology Structural Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Structural Biology Molecular Biology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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